Present address: Department of Plant Pathology, University of California at Davis, One Shields Avenue, Davis, CA 95616-8751, USA.
The pepper RNA-binding protein CaRBP1 functions in hypersensitive cell death and defense signaling in the cytoplasm
Version of Record online: 7 AUG 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 2, pages 235–248, October 2012
How to Cite
Lee, D. H., Kim, D. S. and Hwang, B. K. (2012), The pepper RNA-binding protein CaRBP1 functions in hypersensitive cell death and defense signaling in the cytoplasm. The Plant Journal, 72: 235–248. doi: 10.1111/j.1365-313X.2012.05063.x
- Issue online: 16 OCT 2012
- Version of Record online: 7 AUG 2012
- Accepted manuscript online: 28 MAY 2012 10:05AM EST
- Received 22 August 2011; revised 14 May 2012; accepted 23 May 2012; published online 7 August 2012.
Figure S1. Nucleotide and deduced amino acid sequences of the pepper nucleotide-binding protein CaRBP1. The transcriptional start site is shown in bold type and the asterisk (*) indicates the termination codon. The eukaryotic RNA recognition motif is underlined.
Figure S2. Sequence analysis of CaRBP1 homologs in different plant species. (a) Comparison of the deduced amino acid sequence of the pepper nucleotide-binding protein CaRBP1 with nucleotide-binding proteins from Nicotiana tabacum (accession no. AAC49850), Nicotiana plumbaginifolia (accession no. CAC01238), Lycopersicon esculentum (accession no. AAR91698), and Arabidopsis thaliana (accession no. NP_188544 and BAB02953). Black boxes show identical amino acid residues and gray boxes indicate similar amino acid residues. Gaps introduced to optimize sequence alignments are indicated by dashes (-). (b) Phylogenetic analysis of CaRBP1 homologs from Solanum tuberosum (accession no. ABB86267), Nicotiana tabacum (accession no. AAC49850), Nicotiana plumbaginifolia (accession no. CAC01238), Lycopersicon esculentum (accession no. AAR91698), Vitis vinifera (accession no. CAO47776), Oryza sativa (accession no. NP_001063403), Physcomitrella patens (accession no. EDQ51056), and Arabidopsis thaliana (accession no. AAM67293, NP_188544 and BAB02953). The phylogenetic tree was generated by the MEGA4 program using a neighbor-joining algorithm (Saitou and Nei, 1987; Tamura et al., 2007). The percentages of replicate trees in which the associated taxa clustered together with 3000 bootstrap replicates are indicated next to the branches.
Figure S3. Responses of CaRBP1-OX Arabidopsis plants to Pseudomonas syringae pv. tomato DC3000. (a) RT-PCR analysis of CaRBP1 expression in wild-type and CaRBP1-OX lines. Expression of ACT1 (actin1) was used as a loading control. (b) Bacterial growth in leaves of wild-type and CaRBP1-OX plants 0 or 3 days after inoculation with Pst DC3000 or avrRpm1 (105 cfu mL−1). Data represent the means ± standard deviations from three independent experiments, each with four replicates. Same letters indicate no significant differences, as determined by Fisher’s protected least significant difference (LSD) test (P < 0.05). dai, days after inoculation.
Figure S4. Hyphal growth on cotyledons of 7-day-old wild-type (WT) and CaRBP1-overexpressing Arabidopsis transgenic seedlings (lines #3, #6, and #7) 1, 5 and 7 days after inoculation with Hyaloperonospora arabidopsidis (Hpa) isolate Noco2 (5 × 104 spores mL−1). Infected cotyledons were stained with trypan blue. dai: days after inoculation. Bars = 500 μm.
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