These authors contributed equally to this work.
Targeted inactivation of transcription factors by overexpression of their truncated forms in plants
Article first published online: 19 JUL 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 1, pages 162–172, October 2012
How to Cite
Seo, P. J., Hong, S.-Y., Ryu, J. Y., Jeong, E.-Y., Kim, S.-G., Baldwin, I. T. and Park, C.-M. (2012), Targeted inactivation of transcription factors by overexpression of their truncated forms in plants. The Plant Journal, 72: 162–172. doi: 10.1111/j.1365-313X.2012.05069.x
The authors have no conflict of interest to declare.
- Issue published online: 24 SEP 2012
- Article first published online: 19 JUL 2012
- Accepted manuscript online: 5 JUN 2012 11:40AM EST
- Received 9 April 2012; revised 31 May 2012; accepted 1 June 2012; published online 19 July 2012.
- artificial siPEP;
- peptide interference (PEPi);
- transcription factor;
- technical advance
Transcription factors are central constituents of gene regulatory networks that control diverse aspects of plant development and environmental adaptability. Therefore they have been explored for decades as primary targets for agricultural biotechnology. A gene of interest can readily be introduced into many crop plants, whereas targeted gene inactivation is practically difficult in many cases. Here, we developed an artificial small interfering peptide (a-siPEP) approach, which is based on overexpression of specific protein domains, and evaluated its application for the targeted inactivation of transcription factors in the dicot model, Arabidopsis, and monocot model, Brachypodium. We designed potential a-siPEPs of two representative MADS box transcription factors, SUPPRESSOR OF OVEREXPRESSOR OF CONSTANS 1 (SOC1) and AGAMOUS (AG), and a MYB transcription factor, LATE ELONGATED HYPOCOTYL (LHY). Transgenic plants overproducing the a-siPEPs displayed phenotypes comparable to those of gene-deficient mutants. The a-siPEPs attenuate nuclear import and DNA-binding of target transcription factors. Our data demonstrate that the a-siPEP tool is an efficient genetic means of inactivating specific transcription factors in plants.