These authors contributed equally to this work.
Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion
Article first published online: 24 JUL 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 2, pages 308–319, October 2012
How to Cite
Oh, S. A., Allen, T., Kim, G. J., Sidorova, A., Borg, M., Park, S. K. and Twell, D. (2012), Arabidopsis Fused kinase and the Kinesin-12 subfamily constitute a signalling module required for phragmoplast expansion. The Plant Journal, 72: 308–319. doi: 10.1111/j.1365-313X.2012.05077.x
- Issue published online: 16 OCT 2012
- Article first published online: 24 JUL 2012
- Accepted manuscript online: 18 JUN 2012 11:29AM EST
- Received 25 April 2012; revised 8 June 2012; accepted 12 June 2012; published online 24 July 2012.
- Fused kinase;
- pollen mitosis I;
- Kinesin-12 subfamily;
- phragmoplast expansion
The conserved Fused kinase plays vital but divergent roles in many organisms from Hedgehog signalling in Drosophila to polarization and chemotaxis in Dictyostelium. Previously we have shown that Arabidopsis Fused kinase termed TWO-IN-ONE (TIO) is essential for cytokinesis in both sporophytic and gametophytic cell types. Here using in vivo imaging of GFP-tagged microtubules in dividing microspores we show that TIO is required for expansion of the phragmoplast. We identify the phragmoplast-associated kinesins, PAKRP1/Kinesin-12A and PAKRP1L/Kinesin-12B, as TIO-interacting proteins and determine TIO-Kinesin-12 interaction domains and their requirement in male gametophytic cytokinesis. Our results support the role of TIO as a functional protein kinase that interacts with Kinesin-12 subfamily members mainly through the C-terminal ARM repeat domain, but with a contribution from the N-terminal kinase domain. The interaction of TIO with Kinesin proteins and the functional requirement of their interaction domains support the operation of a Fused kinase signalling module in phragmoplast expansion that depends upon conserved structural features in diverse Fused kinases.