These authors contributed equally to this work.
TECHNICAL ADVANCE / RESOURCE
Tissue-specific profiling of the Arabidopsis thaliana auxin metabolome
Article first published online: 13 AUG 2012
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
The Plant Journal
Volume 72, Issue 3, pages 523–536, November 2012
How to Cite
Novák, O., Hényková, E., Sairanen, I., Kowalczyk, M., Pospíšil, T. and Ljung, K. (2012), Tissue-specific profiling of the Arabidopsis thaliana auxin metabolome. The Plant Journal, 72: 523–536. doi: 10.1111/j.1365-313X.2012.05085.x
- Issue published online: 29 OCT 2012
- Article first published online: 13 AUG 2012
- Accepted manuscript online: 23 JUN 2012 10:48AM EST
- Received 15 March 2012; revised 13 June 2012; accepted 20 June 2012; published online 13 Augut 2012.
- indole-3-acetic acid;
- metabolite profiling;
- Arabidopsis thaliana ;
- technical advance
The plant hormone auxin is believed to influence almost every aspect of plant growth and development. Auxin transport, biosynthesis and degradation combine to form gradients of the hormone that influence a range of key developmental and environmental response processes. There is abundant genetic evidence for the existence of multiple pathways for auxin biosynthesis and degradation. The complexity of these pathways makes it difficult to obtain a clear picture of the relative importance of specific metabolic pathways during development. We have developed a sensitive mass spectrometry-based method to simultaneously profile the majority of known auxin precursors and conjugates/catabolites in small amounts of Arabidopsis tissue. The method includes a new derivatization technique for quantification of the most labile of the auxin precursors. We validated the method by profiling the auxin metabolome in root and shoot tissues from various Arabidopsis thaliana ecotypes and auxin over-producing mutant lines. Substantial differences were shown in metabolite patterns between the lines and tissues. We also found differences of several orders of magnitude in the abundance of auxin metabolites, potentially indicating the relative importance of these compounds in the maintenance of auxin levels and activity. The method that we have established will enable researchers to obtain a better understanding of the dynamics of auxin metabolism and activity during plant growth and development.