SEARCH

SEARCH BY CITATION

Table S1. Primers used for RT-PCR and sequence analysis.

Figure S1. Phenotype of the mpr25 mutant grown in the dark. WT and mpr25 mutants were grown on soil in the dark for 3 weeks. Scale bar = 10 cm.

Figure S2. Effect of light intensity on the chlorophyll fluorescence parameters and P700 redox state in the mpr25 mutant. (a) Non-photochemical quenching (NPQ). (b) Photochemical quenching (qP). (c) Quantum yield of PSII (ΦII). (d) Maximum operating efficiency of PSII (Fv/Fm). (e) Quantum yield of PSI (ΦI). (f) P700 oxidation ratio. *indicates statistically significant differences between WT and mpr25 mutant at P < 0.05 calculated using the Student’s t-test.

Figure S3. Editing and expression of nad5 in various tissues. (a) Direct sequence of the nad5eU1580SL editing site in various tissues. (b) Quantitative RT-PCR analysis of nad5 expression in various tissues. Transcript abundance is indicated relative to that of calli. Quantitative RT-PCR analysis was performed using nad5 gene-specific primers and rrn18 expression was used to normalize the expression values.

Figure S4. nad5 expression in the mpr25 mutant. (a) Quantitative RT-PCR analysis of nad5 expression in etiolated leaves and green leaves of 4-week-old seedlings of WT and the mpr25 mutant. Transcript abundance is indicated relative to that of WT. Quantitative RT-PCR analysis was performed using nad5 gene-specific primers and rrn18 expression was used to normalize expression values. (b) Northern blot analysis of nad5 in etiolated leaves of 4-week-old seedlings of WT and the mpr25 mutant.

Figure S5. Expression analysis of alternative NADH dehydrogenase genes. Quantitative RT-PCR analysis was performed in leaves of four-week-old seedlings using NDA1, NDA2, NDB1, NDB2, NDB3 and NDC1 gene-specific primers. α-tubulin expression was used to normalize expression values. *indicate statistically significant differences between WT and mpr25 mutant at P < 0.05 calculated using the Student’s t-test.

Figure S6. Expression analysis of AOX genes. AOX1a and AOX1c expression in leaves of 4-week-old seedlings was analyzed by quantitative RT-PCR. Transcript abundance is indicated relative to that of WT. *indicate statistically significant differences between WT and mpr25 mutant at P < 0.05 calculated using the Student’s t-test.

Figure S7. SDS-PAGE of purified recombinant MPR25 protein. A molecular size of the purified recombinant MPR25 protein (Trx-rMPR25) used for EMSA was confirmed by SDS-PAGE. The loaded protein was 0.3 mg for Trx-rMPR25 and 1.5 mg for Trx.

As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.

FilenameFormatSizeDescription
tpj5091_sm_FigS1-S7.pdf733KSupporting info item
tpj5091_sm_Supporting-Information-legends.doc30KSupporting info item
tpj5091_sm_TableS1.doc105KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.