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Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions
Article first published online: 19 OCT 2012
DOI: 10.1111/j.1365-313X.2012.05110.x
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd
Additional Information
How to Cite
Kanzaki, H., Yoshida, K., Saitoh, H., Fujisaki, K., Hirabuchi, A., Alaux, L., Fournier, E., Tharreau, D. and Terauchi, R. (2012), Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions. The Plant Journal, 72: 894–907. doi: 10.1111/j.1365-313X.2012.05110.x
Publication History
- Issue published online: 6 DEC 2012
- Article first published online: 19 OCT 2012
- Accepted manuscript online: 17 JUL 2012 07:49PM EST
- Received 6 April 2012; revised 11 July 2012; accepted 13 July 2012; published online 19 October 2012.
Keywords:
- Magnaporthe oryzae ;
- Oryza sativa ;
- avirulence gene;
- resistance gene;
- arms race;
- coevolution;
- population genetics
Summary
Attack and counter-attack impose strong reciprocal selection on pathogens and hosts, leading to development of arms race evolutionary dynamics. Here we show that Magnaporthe oryzae avirulence gene AVR-Pik and the cognate rice resistance (R) gene Pik are highly variable, with multiple alleles in which DNA replacements cause amino acid changes. There is tight recognition specificity of the AVR-Pik alleles by the various Pik alleles. We found that AVR-Pik physically binds the N-terminal coiled-coil domain of Pik in a yeast two-hybrid assay as well as in an in planta co-immunoprecipitation assay. This binding specificity correlates with the recognition specificity between AVR and R genes. We propose that AVR-Pik and Pik are locked into arms race co-evolution driven by their direct physical interactions.