Figure S1. Amino acid sequence alignment of AVR-Pik-A, -B, -C, -D, -E and their ortholog pex75.

Figure S2. Amino acid sequence alignment of Pik-1 CC region corresponding to the positions 6-263 in Costanzo and Jia (2010). The sites 229 and 252 used by Costanzo and Jia for diagnosis of Pik alleles are highlighted.

Figure S3. Rice leaf punch inoculation results of AVR-Pik alleles inoculated to rice line harboring Piks (Shin2-monogenic) and Pikh (K3-monogenic).  Piks interaction is identical to Pikp, and Pikh interaction is identical to Pikm.

Figure S4. Confirmation of expression of bait and prey proteins in yeast-2-hybrid assay by western blot analysis.

Table S1. Geographical distribution of AVR-Pik alleles in the world

Table S2. Rice plant materials used for DNA sequencing analysis of Pik genes.

Table S3. List of the plasmids used in the experiments of cell viability assay in rice protoplast, yeast 2-hybrid assay or in planta co-immunoprecipitation assay.

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