Figure S1. Recombinant MBP-Tab1 protein (aminoacids 390–747). A shorter version of Tab3 (amino acids 390– 747) fused to the maltose binding protein (MBP) wasproduced in E.coli with the pST4-MBP-psaB plasmid. Total proteins  before  and after 1 h of induction with 1mM IPTG (isopropyl β-D-1-thiogalactopyranoside) werefractionated by PAGE and stained with Coomassie Blue. The solublecell extract was loaded on an amylose resin (Biolabs), washed with20 mM Tris-HCl, pH 7.4, 0.2 M NaCl, 10 mM β-mercaptoethanol, 1mM EDTA and eluted with washing buffer containing 10 mM maltose.The four fractions from the elution are labeled 1 – 4. Fraction 2 was used for the RNA binding experiments.

Figure S2. Mutation of tab1-F15. Thefirst base of the CTG codon of L670 is missing in tab1-F15.This deletion creates the stop codon TGA.

Figure S3. Estimation of the amount of Tab1protein. Left: Recombinant Tab1 protein (Rec-Tab1) wasfractionated in decreasing amounts (50, 20, 10, 5, 2.5, 1 and 0 ng)together with 200 µg of total protein fromtab1-F15 by PAGE. Right: Decreasing amounts of totalprotein form wild type (200, 175, 159, 100, 50 and 0 µg) werefractionated with proteins from tab1-F15 so that thetotal amount of protein per lane was 200 µg. After blottingthe filter was reacted with Tab1 antibodies. From the autoradiogramit can be estimates that 2.5 ng of Rec-Tab1 yield a signal ofsimilar strength as that obtained with 200 µg ofwild-type proteins. The amount of Tab1 was estimated asindicated in the text. Authentic Tab1 is indicated with an arrowand recombinant Tab1 protein with a bar. M, size markers.

Table S1. Oligonucleotides.

Table S2. Determination of the essential region of Tab1.

Appendix S1. Determination of the quantity of Tab1 protein.

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