Figure S1. Protein alignment of selected members of the plant PEBP family.

The alignment was created using T-coffee ( Tools/msa/tcoffee/). * amino acids are identical in all sequences in the alignment; conserved substitutions;semi-conserved substitutions; asterisks mark amino acids essential for AtFT vs. AtTFL1 activity (Ahn et al., 2006): LYN-triad defined by Ahn et al. (2006)

Figure S2. Overexpression of NtFT4 and AtFT in tobacco generates an early flowering phenotype. (a – d) The coding region of NtFT4 (a,b) or AtFT (c,d) was inserted downstream of the constitutive Cauliflower mosaic virus 35S promoter and introduced into tobacco by transformation with Agrobacterium tumefaciens.

Figure S3. Branch of the phylogenetic tree to visualize the evolution of FT-like floral repressors. An extended branch of the phylogenetic tree from Figure 1 to visualize the evolution of FT-like floral repressors. Floral repressors are marked in red and floral activators are marked in blue.

Table S1. Protein sequence percentage identities (and similarities) among selected members of the plant PEBP family. Global pairwise alignments were created with Emboss needle ( emboss_needle/).

Table S2. Expression levels determined by qRT-PCR (compared to wild-type expression levels) and growth behavior in T0 plants from each independent transgenic line with a severe phenotype. Time points of bolting and flowering are defined as weeks after the transfer to phytotron (wat). Wild-type plants flowered 4 and 5 wat under LD and SD conditions, respectively.

Table S3. Primer sequences. Underlined nucleotides discriminate between different FT-like genes. Restriction sites are shown in bold. The plus and minus signs indicate primers with and without stop codons. for: forward primer; rev: reverse primer; Tm: annealing temperature.

Table S4. Accession numbers of proteins used for phylogenetic analysis.

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