Anti-D quantification by flow cytometry: an alternative to the Auto Analyser?
Article first published online: 28 JUN 2008
Volume 5, Issue 3, pages 203–208, September 1995
How to Cite
Austin, E. B., McIntosh, Y., Hodson, C. and Lee, D. (1995), Anti-D quantification by flow cytometry: an alternative to the Auto Analyser?. Transfusion Medicine, 5: 203–208. doi: 10.1111/j.1365-3148.1995.tb00229.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 20 February 1995; accepted for publication 10 May 1995
- flow cytometry;
SUMMARY. A flow cytometry method for quantifying levels of anti-D is described. The method is based on the indirect antiglobulin test and uses an FITC Fab anti-human IgG reagent for detection of bound anti-D. A standard curve is generated from the fluorescence measured by flow cytometry using the British Standard anti-D preparation 73/515. The fluorescence obtained from test samples by flow cytometry is converted into IU/mL anti-D, using the standard curve. In a limited study with 42 serum samples the assay showed good correlation with results obtained using the AutoAnalyser for sera with anti-D levels less than 100 IU/mL. The method is simple to perform and has potential as a replacement for the AutoAnalyser.