Serial oxygen equilibrium and kinetic measurements during RBC storage
Article first published online: 31 MAY 2010
Transfusion Medicine © 2010 British Blood Transfusion Society. No claim to original US government works
Volume 20, Issue 5, pages 341–345, October 2010
How to Cite
Gelderman, M. P., Yazer, M. H., Jia, Y., Wood, F., Alayash, A. I. and Vostal, J. G. (2010), Serial oxygen equilibrium and kinetic measurements during RBC storage. Transfusion Medicine, 20: 341–345. doi: 10.1111/j.1365-3148.2010.01016.x
- Issue published online: 6 SEP 2010
- Article first published online: 31 MAY 2010
- Received 4 February 2010; accepted for publication 29 April 2010
- oxygen dissociation;
- rate constants;
- red blood cell;
- storage lesion
Objectives: To contribute to the understanding of the biochemical changes associated with the RBC storage lesion.
Aim: To investigate changes in O2 equilibrium and on/off kinetic rates during routine cold storage.
Background: As RBCs are stored between 1 and 6°C numerous biochemical changes occur within the RBCs, including changes in the properties of the haemoglobin itself. This study serially analysed for the first time the O2 equilibrium and on/off kinetic rates across the RBC membrane during routine storage.
Methods/Materials: The oxygen binding (kon) and offloading (koff) constants were measured in fresh RBCs and then in AS-5-preserved RBCs at weekly intervals, along with oxygen equilibrium curves (OECs), 2,3-Diphosphoglycerate (2,3-DPG), p50 and the Hill number (n).
Results: The kon increased slightly as the 2,3-DPG and p50 decreased during storage, whereas the koff remained largely unchanged. The OECs demonstrated the expected increase in O2 affinity, whereas the Hill number was unchanged during storage.
Conclusion: In spite of the biochemical, structural and functional changes associated with the storage of RBCs, their in vitro interactions with oxygen were largely preserved through 42 days of storage.