Mycobacterium tuberculosis in a HIV-1-infected population from Southeastern Brazil in the HAART era

Authors

  • Heloisa S. P. Pedro,

    1.  Adolfo Lutz Institute –Regional Laboratory from São José do Rio Preto, São Paulo, SP, Brazil
    2.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Maria I. F. Pereira,

    1.  Adolfo Lutz Institute –Regional Laboratory from São José do Rio Preto, São Paulo, SP, Brazil
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  • Maria R. A. Goloni,

    1.  Adolfo Lutz Institute –Regional Laboratory from São José do Rio Preto, São Paulo, SP, Brazil
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  • Fernanda C. Pires,

    1.  Adolfo Lutz Institute –Regional Laboratory from São José do Rio Preto, São Paulo, SP, Brazil
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  • Rosângela S. Oliveira,

    1.  Adolfo Lutz Institute – Central Laboratory from São Paulo, São Paulo, SP, Brazil
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  • Maria A. B. da Rocha,

    1.  DST/AIDS Specialized Attendance Service – São José do Rio Preto, São Paulo, SP, Brazil
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  • Luciana M. Conceição,

    1.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Valéria D. Fraga,

    1.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Juliana C. Fenley,

    1.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Talita Cordeschi,

    1.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Ricardo L. D. Machado,

    1.  Department of Dermatological, Parasitical and Infectious Diseases from Faculty of Medicine from São José do Rio Preto, Center for Microorganisms Investigation, São Paulo, SP, Brazil
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  • Célia Franco,

    1.  Infectious and Parasitical Diseases Service from Hospital de Base – Faculty of Medicine from São José do Rio Preto Foundation, São Paulo, SP, Brazil
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  • Andréa R. B. Rossit

    1.  Microbiology and Parasitical Diseases from Fluminense Federal University, Biomedical Institute, Niterói, RJ, Brazil.
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Corresponding Author Andréa R. B. Rossit, Microbiology and Parasitical Disease Department, Biomedical Institute, Fluminense Federal University, Rua Hernani Pires de Melo, 101, São Domingos, 24210 130 Niterói, RJ, Brazil. E mail: andrea-regina@vm.uff.br

Summary

Objective  To evaluate retrospectively the microbiological profile of Mycobacterium species isolated from HIV-infected patients attending the HIV/TB reference health care units in São José do Rio Preto, Brazil.

Method  Retrospective evaluation of all HIV-1 positive patients whose IAL-SJRP laboratorial analysis was positive for Mycobacterium sp. after diagnosis of HIV Infection, from January 2000 to December 2006.

Results  Of 198 patients, acid-fast staining detected mycobacteria early in 41%. Culture revealed 52.5% to be infected with Mycobacterium tuberculosis (MT). 42.4% had non-tuberculous mycobacteria (NTM) and 5.1% had MT/NTM positive cultures. Eleven per cent of MT strains were resistant to at least one of the antimycobacterial drugs and 3.1% were multidrug resistant. 39.4% of isolated mycobacteria were NTM species.

Conclusion  Our data may serve as a starting point for further comparisons with other Brazilian regions and other developing countries. The data may provide important clues to the future understanding, prevention and control of such co-infections around the world.

Abstract

Objectif:  Evaluer rétrospectivement le profil microbiologique des espèces de Mycobacterium isolées de patients infectés par le VIH fréquentant des unités de soins de santé de référence pour la tuberculose/VIH à São José do Rio Preto, au Brésil.

Méthode:  Evaluation rétrospective de tous les patients VIH-1 positifs dont l’analyse IAL-SJRP de laboratoire était positive pour Mycobacterium sp, de janvier 2000 à décembre 2006.

Résultats:  Sur 198 patients, la coloration acido-résistante a permis de détecter des mycobactéries chez 41% d’eux. La culture a révélé 52,5% infectés par Mycobacterium tuberculosis (MT). 42,4% avaient des mycobactéries non-tuberculeuses (NTM) et 5,1% avaient des cultures positives pour MT et NTM. 11% des souches MT étaient résistantes à au moins un des médicaments antituberculeux, 3,1%étaient multirésistantes. 39,4% des mycobactéries isolées étaient des espèces NTM.

Conclusion:  Nos données pourraient servir de point de départ pour des comparaisons avec d’autres régions du Brésil et d’autres pays en développement. Les données pourraient fournir d’importants indices à la compréhension, prévention et au contrôle de telles coinfections à travers le monde.

Abstract

Objetivo:  Evaluar de forma retrospectiva el perfil microbiológico de las especies de Mycobacterium aisladas de pacientes infectados con VIH atendidos en los centros sanitarios de referencia para VIH/TB en São José do Rio Preto, Brasil.

Método:  Evaluación retrospectiva de todos los pacientes VIH-1 positivos cuyos análisis realizados en el laboratorio IAL-SJRP eran positivos para Mycobacterium sp. tras habérseles diagnosticado la infección por VIH entre Enero 2000 y Diciembre 2006.

Resultados:  Las micobacterias se detectaron mediante tinción ácido-resistente en 41% de 198 pacientes. Los cultivos revelaron que un 52.5% estaban infectados con Mycobacterium tuberculosis (MT). 42.4% tenían una micobacteria no tuberculosa (MNT), 5.1% tenía cultivos MT/MNT positivos. Un 11% de las cepas de MT eran resistentes a al menos uno de los medicamentos antimicobacterianos. Un 39.4% de las micobacterias aisladas eran especies MNT.

Conclusiones:  Nuestros datos podrían servir como punto de partida para futuras comparaciones con otras regiones del Brasil y de otros países en vías de desarrollo. Los datos podrían proveer claves importantes para la comprensión futura, prevención y control de este tipo de coinfecciones alrededor del mundo.

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