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Apoptosis and anti-apoptotic heat shock proteins in canine cutaneous infundibular keratinizing acanthomas and squamous cell carcinomas

Authors

  • Laura Bongiovanni,

    1. Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45–64100 Teramo, Italy
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  • Mariarita Romanucci,

    1. Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45–64100 Teramo, Italy
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  • Pierluigi Fant,

    1. Laboratoire de Histo-cytopathologie Vétérinaire de Maisons-Alfort, rue Raspail –94700 Maisons-Alfort, France
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  • Marie Lagadic,

    1. Laboratoire de Histo-cytopathologie Vétérinaire de Maisons-Alfort, rue Raspail –94700 Maisons-Alfort, France
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  • Leonardo Della Salda

    1. Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45–64100 Teramo, Italy
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  • The present work was presented in a talk at the 23rd Meeting of the European Society of Veterinary Pathology – Naples 7/10 September 2005 and published as an abstract in the Proceedings of the 23rd Meeting of the European Society of Veterinary Pathology (ESVP), Naples (Italy), 7/10 September 2005, p. 37.

Prof Leonardo Della Salda, Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro, 45-64100 Teramo, Italy.
E-mail: ldellasalda@unite.it

Abstract

Cell stress and death are linked in the neoplastic process, and heat shock proteins appear to play an important role by inhibiting apoptotic pathways. The apoptotic rates in 9 canine infundibular keratinizing acanthomas (IKAs) and 17 canine squamous cell carcinomas (SCCs) were correlated with the immunohistochemical expression of caspase-3 and the antiapoptotic heat shock proteins Hsp27, 72 and 73. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) method.

The absence of a correlation between the TUNEL index and active-caspase-3 expression, a paucity of active-caspase-3-positive cells and Hsp72 over-expression were considered to be indicative of inhibition of apoptosis, and suggestive that inhibition of cell death plays a key role in oncogenesis and tumour growth of some canine skin neoplasms.

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