Source of Funding Funding for this study was provided by Greer Laboratories Inc. Conflict of Interest Kenneth W. Lee, Karen D. Blankenship, Zachary M. McCurry, and Robert E. Esch are employees at Greer Laboratories Inc. Douglas J. DeBoer and Rosanna Marsella serve as members of Greer's Veterinary Scientific Advisory Board.
Performance characteristics of a monoclonal antibody cocktail-based ELISA for detection of allergen-specific IgE in dogs and comparison with a high affinity IgE receptor-based ELISA
Article first published online: 3 APR 2009
© 2009 The Authors. Journal compilation © 2009 ESVD and ACVD
Volume 20, Issue 3, pages 157–164, June 2009
How to Cite
Lee, K. W., Blankenship, K. D., McCurry, Z. M., Esch, R. E., DeBoer, D. J. and Marsella, R. (2009), Performance characteristics of a monoclonal antibody cocktail-based ELISA for detection of allergen-specific IgE in dogs and comparison with a high affinity IgE receptor-based ELISA. Veterinary Dermatology, 20: 157–164. doi: 10.1111/j.1365-3164.2009.00740.x
- Issue published online: 18 MAY 2009
- Article first published online: 3 APR 2009
- Accepted 11 November 2008
The purpose of this study was to define the operational and performance characteristics of a commercially available monoclonal antibody based (mac) ELISA for detection of allergen-specific IgE in dogs. The average intra-assay variance over 1 year was 9.7% (range 2.5–62.7%), while the interassay variance averaged 10.8% (range 8.1–13.8%). The average positive control responses observed for grass, weed, tree and mite allergens during each month remained relatively constant; the average monthly variance was 11.6% (range 8.3–19.2%) for grass pollens, 13.3% (range 9.1–20.4%) for weed pollens, 13.3% (range 9.8–18.2%) for tree pollens and 13.6% (range 8.9–18.7%) for mite allergens. The interlaboratory concordance of results for the macELISA was approximately 91%. The interlaboratory concordance of results comparing the macELISA and a high affinity IgE receptor-based ELISA was approximately 92%. The results demonstrate that the macELISA is reproducible and the results are comparable to the high affinity IgE receptor based ELISA within and between laboratories.