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Performance characteristics of a monoclonal antibody cocktail-based ELISA for detection of allergen-specific IgE in dogs and comparison with a high affinity IgE receptor-based ELISA

Authors


  • Source of Funding
    Funding for this study was provided by Greer Laboratories Inc.
    Conflict of Interest
    Kenneth W. Lee, Karen D. Blankenship, Zachary M. McCurry, and Robert E. Esch are employees at Greer Laboratories Inc. Douglas J. DeBoer and Rosanna Marsella serve as members of Greer's Veterinary Scientific Advisory Board.

Kenneth W. Lee, PhD, Greer Laboratories, 639 Nuway Circle, Lenoir, NC 28645, USA. E-mail: klee@greerlabs.com

Abstract

The purpose of this study was to define the operational and performance characteristics of a commercially available monoclonal antibody based (mac) ELISA for detection of allergen-specific IgE in dogs. The average intra-assay variance over 1 year was 9.7% (range 2.5–62.7%), while the interassay variance averaged 10.8% (range 8.1–13.8%). The average positive control responses observed for grass, weed, tree and mite allergens during each month remained relatively constant; the average monthly variance was 11.6% (range 8.3–19.2%) for grass pollens, 13.3% (range 9.1–20.4%) for weed pollens, 13.3% (range 9.8–18.2%) for tree pollens and 13.6% (range 8.9–18.7%) for mite allergens. The interlaboratory concordance of results for the macELISA was approximately 91%. The interlaboratory concordance of results comparing the macELISA and a high affinity IgE receptor-based ELISA was approximately 92%. The results demonstrate that the macELISA is reproducible and the results are comparable to the high affinity IgE receptor based ELISA within and between laboratories.

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