Figure S1. Amino acid sequence of the canine IL-31 construct. The identity of purified canine IL-31 protein was confirmed using mass spectrometry to analyze a tryptic digest of the protein and also by N-terminal sequencing.

Figure S2. Detection of phospho-Akt in IL-31-treated DH82 cells. Using Western blotting techniques, phospho-Akt levels were evaluated in DH82 cells treated with varying concentrations of IL-31 (0–10 ug/mL).

Table S1. Individual IL-31 serum levels in dogs with atopic dermatitis. Protein levels of IL-31 were measured in serum using immunoassay techniques in client-owned animals diagnosed with atopic dermatitis. Quantitative levels of IL-31 in serum are listed for each dog evaluated.

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