Steroid-induced Dermal Atrophy

Effects of Glucocorticosteroids on Collagen Metabolism in Human Skin Fibroblast Cultures

Authors

  • Barbara A. Booth Ph.d.,

    1. From the Division of Dermatology, Department of Medicine, University of California at Los Angeles School of Medicine, Harbor-UCLA Medical Center, Torrance, California, and the Division of Dermatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri
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  • Elaine M. L. Tan M.Sc.,

    1. From the Division of Dermatology, Department of Medicine, University of California at Los Angeles School of Medicine, Harbor-UCLA Medical Center, Torrance, California, and the Division of Dermatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri
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  • Aarne Oikarinen M.D.-Ph.D.,

    1. From the Division of Dermatology, Department of Medicine, University of California at Los Angeles School of Medicine, Harbor-UCLA Medical Center, Torrance, California, and the Division of Dermatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri
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  • Jouni Uitto M.D.-Ph.D.

    Corresponding author
    1. From the Division of Dermatology, Department of Medicine, University of California at Los Angeles School of Medicine, Harbor-UCLA Medical Center, Torrance, California, and the Division of Dermatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri
      Address for reprints; jouni Uitto, M.D.-Ph.D., Division of Dermatology, Harbor-UCLA Medical Center, 1000 West Carson Street, Torrance, CA 90509.
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  • Supported by USPHS, NIH grants CM 28833, AM 28450, and AM 07284. Dr. Uitto is recipient of Research Career Development Award 5KO4 AM00897.

Address for reprints; jouni Uitto, M.D.-Ph.D., Division of Dermatology, Harbor-UCLA Medical Center, 1000 West Carson Street, Torrance, CA 90509.

Abstract

ABSTRACT: Collagen production by human skin fibroblast cultures was studied by incubation with [3H] proline and several glucocorticosteroids known to produce dermal atrophy in vivo. Collagen production was measured as formation of [3H]hydroxyproline or collagenase-digestible3H-polypeptides, and the values were corrected for changes in cell number in the same cultures. The steroids, in a wide concentration range, failed to elicit any consistent alterations in collagen production. Review of the literature dealing with corticosteroid-induced changes in collagen production by human skin fibroblasts indicate conflicting results even under apparently similar incubation conditions. Consequently, no unifying hypothesis for steroid-induced dermal atrophy can be developed presently based on the in vitro data.

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