A peptide carrier for the delivery of elastin into fibroblast cells

Authors

  • Saman Ahmad Nasrollahi PHARMD,

    1. Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
    2. Molecular Research Lab, Department of Pharmacology and Toxicology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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  • Shamileh Fouladdel PHARMD,

    1. Molecular Research Lab, Department of Pharmacology and Toxicology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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  • Changiz Taghibiglou PHARMD, PhD,

    1. Department of Pharmacology, University of Saskatchewan, Saskatoon, Canada
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  • Ebrahim Azizi PHARMD, PhD,

    1. Molecular Research Lab, Department of Pharmacology and Toxicology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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  • Effat Sadat Farboud PHARMD, PhD

    1. Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
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  • Conflicts of interest: None.

Dr. Effat Sadat Farboud, pharmd, phD
Department of Pharmaceutics, School of Pharmacy
Tehran University of Medical Sciences
Enghelab Avenue
Tehran
Iran
E-mail: farbod@sina.tums.ac.ir

Abstract

Background  Transmembrane delivery of active peptides and proteins, including skin delivery of cosmeceutical proteins such as collagen and elastin, has been a challenging issue. Amphipathic cell-penetrating peptides (CPPs) have been proposed as carrier peptides to mediate cellular uptake of proteins without covalent binding.

Materials and methods  In this study, we have used a short peptide, Pep-1, as our CPP to transport elastin into fibroblast cells. Different ratios of Pep-1/elastin complexes were produced by using a fixed amount of elastin and different molar ratio of Pep-1. The ability of transduction into cells was determined by fluorescence microscopy. The characteristics of Pep-1/elastin complexes were monitored using scanning electron microscopy and photon correlation spectroscopy.

Results  No cellular toxicity was observed in cells treated with Pep-1/elastin complex. Finally, we determined a Pep-1 : elastin ratio of 10 : 1 as the most effective ratio in cellular delivery of elastin.

Conclusion  Pep-1 mediated fast and effective delivery of elastin as a cosmetic protein into fibroblast cells in the treatment of skin-aging symptoms.

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