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Oxymatrine inhibits collagen synthesis in keloid fibroblasts via inhibition of transforming growth factor-β1/Smad signaling pathway
Conflicts of interest: We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work.
Yu-Xin Wang, md
Department of Plastic Surgery
First Affiliated Hospital of China Medical University
North 2nd Road, Heping District
Shenyang 110001
Liaoning China
E-mail: cmuwyx6431@yahoo.com
Abstract
Background Keloids are benign dermal tumors characterized by fibroblastic proliferation and excessive accumulation of collagen. Oxymatrine (OMT) is an alkaloid extracted from the Chinese herb Sophora japonica with capacities of anti-fibrosis.
Objective To evaluate the effects of OMT on collagen production and to explore its mechanisms.
Methods OMT was applied to human keloid fibroblasts in vitro. Collagen, transforming growth factor (TGF)-β1, TGF-β receptor, and Smads were analyzed by Western Blot, reverse transcription polymerase chain reaction, and immunofluorescence.
Results We found that both collagen synthesis and Smad3 production were significantly suppressed in a dose-dependent administration of OMT. However, expression of TGF-β1, TGF-β receptor1, TGF-β receptor2, Smad4, and Smad7 was unchanged. We also found that OMT reversed phosphorylation and nuclear translocation of Smad3 induced by TGF-β1.
Conclusions OMT inhibited collagen synthesis, which might be associated with TGF-β/Smad signaling pathway. These findings suggest that OMT may be a promising candidate to prevent keloid and other fibrotic diseases.