Effects of Chloroquine, Mefloquine and Quinine on Natural Killer Cell Activity in vitro

An Analysis of the Inhibitory Mechanism


Bente K Pedersen, M.D., Laboratory of Medical Immunology, 7544, Tagensvej 20, Rigshospitalet, DK-2200 Copenhagen N, Denmark


Natural killer (NK) cell activity against K 562 target cells was inhibited by pharmacological concentrations of chloroquine, mefloquine and quinine. The most potent were mefloquine and quinine. The drug-induced inhibition of the NK cell activity was abolished by addition of α interferon (IF) or interleukin 2 (II-2); preincubation of mononuclear cells with IF or II-2 followed by addition of anti-malarial drugs decreased the inhibitory effects of the drugs. The drug-induced inhibition of the NK cell activity was not dependent on the presence of monocytes. Using monocyte depleted Percoll fractionated NK cell enriched populations in a single cell agarose assay, it was shown that the inhibitory effects of mefloquine, but not of chloroquine and quinine were due to an inhibition of the formation of effector/target cell conjugates.