Immunoblotting analysis of concanavalin A -isolated allergens of Candida albicans


Johannes Savolaine, Department of Medical Microbiology, University of Turku, 20520 Turku, Finland


The carbohydrate-containing Traction of Candida albicans was isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE-immuno-blotting with individual and pooled sera from C. albicans-allergic subjects. In the ConA-bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA-bound mannose-containing fraction of C. albicans.