IgE binding to pepsin-digested water soluble and insoluble wheat proteins
Version of Record online: 5 AUG 2004
Volume 59, Issue 11, pages 1229–1232, November 2004
How to Cite
Mesa-del-Castillo, M., Martínez-Cócera, C., Caballero, M. L., Vazquez, L. and Moneo, I. (2004), IgE binding to pepsin-digested water soluble and insoluble wheat proteins. Allergy, 59: 1229–1232. doi: 10.1111/j.1398-9995.2004.00447.x
- Issue online: 5 AUG 2004
- Version of Record online: 5 AUG 2004
- Accepted for publication 22 October 2003
Background: Positive skin prick tests to wheat are a common finding among atopic patients, but only a minor fraction of these patients show immediate clinical symptoms after wheat ingestion. The aim of this study was to evaluate the allergenicity of wheat proteins after pepsin treatments.
Methods: Six patients with positive specific IgE and/or skin prick test to soluble wheat proteins and to gluten were studied. Five of them had no symptoms after wheat ingestion and showed a negative oral challenge test. All sera were analysed with extracts obtained after pepsin hydrolysis during different time periods of water-soluble and insoluble wheat proteins by IgE immunoblotting.
Results: A pepsin-sensitive allergen of around 35 kDa was recognized by the five atopic negative wheat oral challenge patients in the undigested water-insoluble extract. A patient showing immediate urticaria after wheat ingestion detected a pepsin-resistant allergen with similar molecular weight. Finally, an inhalation-induced asthma patient recognized water-soluble proteins (of around 14 kDa) that could correspond to the α-amylase inhibitors.
Conclusions: Our immunoblotting method shows better correlation with the clinical symptoms than skin prick test and CAP results. Pepsin resistance of the acetic acid-soluble wheat proteins may be responsible for the immediate symptoms of the patient who presented immediate urticaria.