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Identification of tryptase- and chymase-related gene clusters in human mast cells using microarrays

Authors

  • C. Dahl,

    1. 1 A Research, Department of Pediatrics, Aarhus University Hospital, Aarhus, Denmark ; 2 Research Unit for Allergy Transcriptome, RIKEN Research Center for Allergy and Immunology, Tokyo, Japan ; 3 Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan ; 4 Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
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  • 1 H. Saito,

    1. 1 A Research, Department of Pediatrics, Aarhus University Hospital, Aarhus, Denmark ; 2 Research Unit for Allergy Transcriptome, RIKEN Research Center for Allergy and Immunology, Tokyo, Japan ; 3 Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan ; 4 Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
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  • 2 M. Kruhøffer,

    1. 1 A Research, Department of Pediatrics, Aarhus University Hospital, Aarhus, Denmark ; 2 Research Unit for Allergy Transcriptome, RIKEN Research Center for Allergy and Immunology, Tokyo, Japan ; 3 Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan ; 4 Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
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  • and 3 P. O. Schiøtz 4

    1. 1 A Research, Department of Pediatrics, Aarhus University Hospital, Aarhus, Denmark ; 2 Research Unit for Allergy Transcriptome, RIKEN Research Center for Allergy and Immunology, Tokyo, Japan ; 3 Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan ; 4 Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
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Christine Dahl
A Research
Department of Pediatrics
Aarhus University Hospital
Brendstrupgaardsvej 100
8200 Aarhus N
Denmark

Abstract

Tryptase and chymase are the two major granular proteases present in human mast cell (MC)s. We used oligonucleotide microarray to measure the levels of approximately 22 000 transcripts in cord blood-derived MCs at 4 weeks, 8 weeks, 12 weeks and 18 weeks in culture. Tryptase (TPSB2) was expressed at the highest level among all transcripts and its expression level reached a plateau at 8 weeks. On the other hand, the expression level of chymase (CMAI) doubled every 4–6 weeks. A similar tendency was found at the protein levels with FACS analysis. After filtering the transcripts with MC-specificity, hierarchical clustering analysis identified 494 and 81 transcripts in the same clusters with tryptase and chymase, respectively. MC-specific genes, KIT and HDC were found in the tryptase cluster. In the chymase cluster, a critical suppressor for cell senescence, BMI1 and the several related genes were found, suggesting that chymase expression may be closely related to cell senescence/quiescence events.

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