• basophil activation;
  • Basotest;
  • FastImmune;
  • flow cytometry;
  • grass pollen

Background:  Two-flow cytometry methods for quantification of degranulated basophil after allergen-specific activation were discussed. The methods are discerned by used membrane receptors – FcɛRI or IL-3Rα Our goal was to evaluate the diagnostic potential of the methods and to correlate them to allergen-specific IgE detection and skin prick test (SPT).

Methods:  Patient's and control's groups were studied with Bulgarian grass pollen allergen B1 simultaneously by flow cytometry kits: Basotest and BD FastImmune test. Allergy diagnosis was based on clinical history and SPT. The determination of specific IgE was performed by ELISA - RIDASCREENR.

Results:  There were no significant differences between the patient's results from Basotest and FastImmune (P > 0.05). A significant correlation between values, analyzed by Basotest and by FastImmune was found (r = 0.88). The sensitivity and specificity of the Basotest, FastImmune, specific IgE and SPT were 85%, 72%, 92% and 92% sensitivity and 100%, 92%, 100% and 85% specificity respectively. The efficiency was between 82% and 97%. There were a significant correlation between the specific IgE and flow cytometry tests: tau = 0.92 (Basotest) and tau = 0.71 (FastImmune) and a moderate significant correlation between the SPT and the in vitro tests: tau = 0.26 (Basotest) and tau = 0.31 (FastImmune).

Conclusion:  The successful use of the Bulgarian grass pollen allergen B1 and both flow cytometry tests was presented. These methods could be as specific tools for IgE-mediated diagnosis especially FastImmune in the case of low IgE receptor expression.