Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps
Article first published online: 6 DEC 2006
Volume 62, Issue 1, pages 66–72, January 2007
How to Cite
Chen, Y.-S., Langhammer, T., Westhofen, M. and Lorenzen, J. (2007), Relationship between matrix metalloproteinases MMP-2, MMP-9, tissue inhibitor of matrix metalloproteinases-1 and IL-5, IL-8 in nasal polyps. Allergy, 62: 66–72. doi: 10.1111/j.1398-9995.2006.01255.x
- Issue published online: 6 DEC 2006
- Article first published online: 6 DEC 2006
- Accepted for publication 11 September 2006
- matrix metalloproteinase;
- nasal polyps;
- tissue inhibitor of matrix metalloproteinase
Background: Nasal polyps (NP), a subgroup of chronic rhinosinusitis, are characterized by interleukin 5 (IL-5) mediated infiltration of eosinophils in sinus mucosa, leading to pseudostratified ciliated columnar epithelium, thickening of the epithelial basement membrane and tissue edema. Matrix metalloproteinases (MMP) constitute a large group of Zn2+ dependent endopeptidases with the ability to degrade extracellular matrix and are possibly responsible for the development of tissue edema in chronic sinusitis.
Objective: The aim of this study was to determine the expression of MMP-2, MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA and to locate the distribution of MMP-2, MMP-9 and TIMP-1 by immunohistochemistry in ethmoid sinus mucosa in NP. Furthermore the correlation between IL-5 or IL-8 and MMP-2, MMP-9 or TIMP-1 is examined.
Methods: Nasal polyps of 33 patients and 18 specimens of inferior turbinate mucosa were examined by real time RT-PCR for MMP-2, MMP-9, TIMP-1, IL-5 and IL-8 mRNA expression. Immunohistochemical labeling for MMP-2, MMP-9 and TIMP-1 was performed.
Results: Differences between both locations were detectable for MMP-9 (P < 0.001) and IL-5 (P = 0.003) but not for MMP-2 (P = 0.278), TIMP-1 (P = 0.515) and IL-8 (P = 0.386). Correlation was detected only between TIMP-1 and IL-5 (r = 0.422, P = 0.014). Cytoplasmic staining of MMP-2 was present in the apical part of the ciliated cells, submucosal glands and in smooth muscle cells. Matrix metalloproteinase-9 was expressed in surface epithelium, in seromucous glands and in polymorphonuclear cells.
Conclusions: Expression of MMP-9 and IL-5 mRNA are associated with NP. The correlation between IL-5 and TIMP-1 indicates the role of TIMP-1 in maintaining the homeostasis in NP.