Original article: Myrmecia pilosula (Jack Jumper) ant venom: identification of allergens and revised nomenclature

Authors

  • M. D. Wiese,

    1. Department of Pharmacy, Royal Hobart Hospital, Hobart
    2. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide
    3. Department of Pharmacy, Flinders Medical Centre, Bedford Park
    4. School of Medicine, University of Tasmania, Hobart
    Search for more papers by this author
  • S. G. A. Brown,

    1. Department of Medicine, Royal Hobart Hospital, Hobart
    2. Discipline of Emergency Medicine, University of Western Australia, Fremantle
    3. Division of Molecular Biotechnology, Telethon Institute for Child Health Research, Subiaco
    Search for more papers by this author
  • T. K. Chataway,

    1. School of Medicine, Flinders University of South Australia, Adelaide
    Search for more papers by this author
  • N. W. Davies,

    1. Central Science Laboratory, University of Tasmania, Sandy Bay
    Search for more papers by this author
  • R. W. Milne,

    1. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide
    Search for more papers by this author
  • S. J. Aulfrey,

    1. Discipline of Emergency Medicine, University of Western Australia, Fremantle
    2. Division of Molecular Biotechnology, Telethon Institute for Child Health Research, Subiaco
    Search for more papers by this author
  • R. J. Heddle

    1. School of Medicine, Flinders University of South Australia, Adelaide
    2. Department of Respiratory Medicine, Flinders Medical Centre, Bedford Park, Australia
    Search for more papers by this author

Michael D. Wiese
Pharmacy Department
Royal Hobart Hospital
GPO Box 1061L
Hobart 7001
Tasmania
Australia

Abstract

Background:  The ‘Jack Jumper Ant’ (JJA; Myrmecia pilosula species complex) is the major cause of ant sting anaphylaxis in Australia. Our aims were to determine the allergenicity of previously described venom peptides in their native forms, identify additional allergens and if necessary, update nomenclature used to describe the allergens according to International Union of Immunological Societies criteria.

Methods:  Various polyacrylamide gel electrophoresis methods were used to separate JJA venom. Gel resolved venom was Western-blotted and probed with individual sera taken from patients with a history of JJA sting anaphylaxis and immunoglobulin E radioallergosorbent test (IgE RAST) tracer uptakes of >1% to whole venom.

Results:  Of 67 available sera, 54 had RAST uptakes >1%. Thirteen IgE binding bands were identified using these sera. Pilosulin 3, [Ile5]pilosulin 1, and pilosulin 4.1 were recognized by 42 (78%), 18 (33%) and nine (17%) of the 54 sera that were tested. Immunoglobulin E-binding proteins with estimated molecular masses of 6.6, 22.8, 25.6, 30.4, 32.1, 34.4 and 89.8 kDa were each recognized by three or more individual sera. Two of these (25.6 and 89.8 kDa) were recognized by 46% and 37% of sera, respectively.

Conclusion:  Nomenclature used to describe JJA venom allergens has been revised. Pilosulin 3 (Myr p 2) is the only major allergen, whilst [Ile5]pilosulin 1 (Myr p 1), and pilosulin 4.1 (Myr p 3) are minor allergens. There are an additional five IgE-binding proteins that require further characterization before they can be named as allergens. These findings provide a framework for standardizing venom extracts for diagnosis and immunotherapy.

Ancillary