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Utility of the lymphocyte transformation test in the diagnosis of drug sensitivity: dependence on its timing and the type of drug eruption
Article first published online: 30 OCT 2007
DOI: 10.1111/j.1398-9995.2007.01553.x
Additional Information
How to Cite
Kano, Y., Hirahara, K., Mitsuyama, Y., Takahashi, R. and Shiohara, T. (2007), Utility of the lymphocyte transformation test in the diagnosis of drug sensitivity: dependence on its timing and the type of drug eruption. Allergy, 62: 1439–1444. doi: 10.1111/j.1398-9995.2007.01553.x
Publication History
- Issue published online: 30 OCT 2007
- Article first published online: 30 OCT 2007
- Accepted for publication 18 August 2007
- Abstract
- Article
- References
- Cited By
Keywords:
- DIHS;
- DRESS;
- drug reaction;
- lymophocyte transformation test;
- SJS;
- toxic epidermal necrolysis
Background: Lymphocyte transformation test (LTT) is a safety and reproducible test to assess activation of drug-specific T cells in vitro; however, there are several practical concerns such as the time of testing and the influence of treatment. Our aim was to define the right timing to perform LTT for determining the causative agent in various types of drug reactions.
Methods: Lymphocyte transformation test was performed at different time points during the evolution of three types of drug reactions, maculo-papular type of drug eruptions (MP), Stevens–Johnson syndrome/toxic epidermal necrolysis (SJS/TEN), and drug-induced hypersensitivity syndrome/drug rash and eosinophilia with systemic symptoms (DIHS/DRESS).
Results: Positive LTT reactions were obtained when the test was performed at the acute stage but not the recovery stage in MP and SJS/TEN, while positive LTT reactions were obtained at the recovery stage but not the acute stage in DIHS/DRESS, regardless of treatment with systemic prednisolone.
Conclusions: Lymphocyte transformation test is a reliable method to define the causative agent, when LTT is performed at the right timing depending on the type of drug reactions. Lymphocyte transformation test should be performed within 1 week after the onset of skin rashes in patients with MP and SJS/TEN; and 5–8 weeks after in patients with DIHS/DRESS, respectively.