You have full text access to this OnlineOpen article

Anti-proliferative and anti-remodelling effect of beclomethasone dipropionate, formoterol and salbutamol alone or in combination in primary human bronchial fibroblasts

  1. D. Descalzi1,†,
  2. C. Folli1,†,
  3. G. Nicolini2,
  4. A. M. Riccio1,
  5. C. Gamalero1,
  6. F. Scordamaglia1,
  7. G. W. Canonica1

Article first published online: 10 DEC 2007

DOI: 10.1111/j.1398-9995.2007.01582.x

Issue

Allergy

Allergy

Volume 63, Issue 4, pages 432–437, April 2008

Additional Information

How to Cite

Descalzi, D., Folli, C., Nicolini, G., Riccio, A. M., Gamalero, C., Scordamaglia, F. and Canonica, G. W. (2008), Anti-proliferative and anti-remodelling effect of beclomethasone dipropionate, formoterol and salbutamol alone or in combination in primary human bronchial fibroblasts. Allergy, 63: 432–437. doi: 10.1111/j.1398-9995.2007.01582.x

Author Information

  1. 1

    Allergy and Respiratory Diseases, Department of Internal Medicine (DIMI), University of Genoa, Genoa

  2. 2

    Medical Department, Chiesi Farmaceutici S.p.A., Parma, Italy

  1. †

    Both the authors contributed equally to this work.

*Giorgio Walter Canonica, MD Allergy and Respiratory Diseases Department of Internal Medicine (DIMI) University of Genoa Largo Rosanna Benzi 10 Pad Maragliano 16132 Genova Italy

  • †

    Both the authors contributed equally to this work.

Publication History

  1. Issue published online: 19 DEC 2007
  2. Article first published online: 10 DEC 2007
  3. Accepted for publication 5 October 2007

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (191K)

References

  • 1
    Global Initiative for Asthma. Global strategy for asthma management and prevention. NHLBI/WHO Workshop Report. Bethesda, MD: National Heart, Lung and Blood Institute, 2002. NIH Publication, N 02-3659: 1–197.
  • 2
    Lange P, Parner J, Vestbo J, Schnohr P, Jensen G. A 15-year follow-up study of ventilatory function in adults with asthma. N Engl J Med 1998;339:1194–1200.
  • 3
    Fabbri LM, Romagnoli M, Corbetta L, Casoni G, Busljetic K, Turato G et al. Differences in airway inflammation in patients with fixed airflow obstruction due to asthma or chronic obstructive pulmonary disease. Am J Respir Crit Care Med 2003;167:418–424.
  • 4
    Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma. From bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med 2000;161:1720–1745.
  • 5
    Greening AP, Ind PW, Northfield M, Shaw G. Added salmeterol versus higher-dose corticosteroid in asthma patients with symptoms on existing inhaled corticosteroid. Allen & Hanburys Limited UK Study Group. Lancet 1994;344:219–224.
  • 6
    Van Noord JA, Schreurs AJ, Mol SJ, Mulder PG. Addition of salmeterol versus doubling the dose of fluticasone propionate in patients with mild to moderate asthma. Thorax 1999;54:207–212.
  • 7
    Papi A, Canonica GW, Maestrelli P, Paggiaro P, Olivieri O, Pozzi E et al. Rescue use of beclomethasone and albuterol in a single inhaler for mild asthma. N Engl J Med 2007;356: 2040–2052.
  • 8
    Eda R, Sugiyama H, Hopp RJ, Okada C, Bewtra AK, Townley RG. Inhibitory effects of formoterol on platelet-activating factor induced eosinophil chemotaxis and degranulation. Int Arch Allergy Immunol 1993;102:391–398.
  • 9
    Mita H, Shida T. Anti-allergic activity of formoterol, a new beta-adrenoceptor stimulant, and salbutamol in human leukocytes and human lung tissue. Allergy 1983;38:547–552.
    Direct Link:
  • 10
    Caramori G, Adcock IM. Pharmacology of airway inflammation in asthma and COPD. Pulm Pharmacol Ther 2003;16:247–277.
  • 11
    Johnson M. Effects of beta2-agonists on resident and infiltrating inflammatory cells. J Allergy Clin Immunol 2002;110:S282–S290.
  • 12
    Oddera S, Silvestri M, Sacco O, Lantero S, Morelli MC, Rossi GA. Evaluation of the inhibitory effects of budesonide on the mitogen-induced or the allergen-induced activation of blood mononuclear cells isolated from asthmatics patients. Ann Allergy Asthma Immunol 1995;75:33–40.
  • 13
    Linden M, Brattsand R. Effects of a corticosteroid, budesonide, on alveolar macrophage and blood monocyte secretion of cytokines: differential sensitivity of GM-CSF, IL-1ß and IL-6. Pulm Pharmacol 1994;7:43–47.
  • 14
    Miyamasu M, Misaki Y, Izumi S, Takaishi T, Morita Y, Nakamura H et al. Glucocorticoids inhibit chemokine generation by human eosinophils. J Allergy Clin Immunol 1998;101:75–83.
  • 15
    Mullol J, Xaubet A, Lopez E, Roca-Ferrer J, Picado C. Comparative study of the effects of different gluco-corticosteroids on eosinophil survival primed by cultured epithelial cell supernatants obtained from nasal mucosa and nasal polyps. Thorax 1995;50:270–274.
  • 16
    Barnes PJ, Adcock IM. How do corticosteroids work in asthma? Ann Intern Med 2003;139:359–370.
  • 17
    Spoelstra FM, Postma DS, Hovenga H, Noordhoek JA, Kauffman HF. Budesonide and formoterol inhibit ICAM-1 and VCAM-1 expression of human lung fibroblasts. Eur Respir J 2000;15:68–74.
  • 18
    Oddera S, Cagnoni F, Mangraviti S, Giron-Michel J, Popova O, Canonica GW. Effects of triamcinolone acetonide on adult human lung fibroblasts: decrease in proliferation, surface molecule expression and mediator release. Int Arch Allergy Immunol 2002;129:152–159.
  • 19
    Leis M, Marschall M, Stamminger T Downregulation of the cellular adhesion molecule Thy-1 (CD90) by cytomegalovirus infection of human fibroblasts. J Gen Virol 2004;85:1995–2000.
  • 20
    Pure E, Cuff CA. A crucial role for CD44 in inflammation. Trends Mol Med 2001;7:213–221.
  • 21
    Teder P, Vandivier RW, Jiang D, Liang J, Cohn L, Puré E et al. Resolution of lung inflammation by CD44. Science 2002;296:155–158.
  • 22
    Sacco O, Silvestri M, Sabatini F, Sale R, Defilippi AC, Rossi GA. Epithelial cells and fibroblasts: structural repair and remodelling in the airways. Paediatr Respir Rev 2004;5(Suppl. A):S35–S40.
  • 23
    Cazes E, Giron-Michel J, Baouz S, Doucet C, Cagnoni F, Oddera S et al. Novel anti-inflammatory effects of the inhaled corticosteroid fluticasone propionate during lung myofibroblastic differentiation. J Immunol 2001;167:5329–5337.
  • 24
    Profita M, Gagliardo R, Di Giorgi R, Pompeo F, Gjomarkaj M, Nicolini G et al. Biochemical interaction between effects of beclomethasone dipropionate and salbutamol or formoterol in sputum cells from mild to moderate asthmatics. Allergy 2005;60:323–329.
    Direct Link:
  • 25
    Doucet C, Brouty-Boye D, Pottin-Clemenceau C, Jasmin C, Canonica GW, Azzarone B. IL-4 and IL-13 specifically increase adhesion molecule and inflammatory cytokine expression in human lung fibroblasts. Int Immunol 1998;10:1421–1433.
  • 26
    Doucet C, Brouty-Boye D, Pottin-Clemenceau C, Canonica GW, Jasmin C, Azzarone B. Interleukin (IL) 4 and IL-13 act on human lung fibroblasts. Implication in asthma. J Clin Invest 1998;101:2129–2139.
  • 27
    Baouz S, Giron-Michel J, Azzarone B, Giuliani M, Cagnoni F, Olsson S et al. Lung myofibroblasts as targets of salmeterol and fluticasone propinate: inhibition of alpha-SMA and NF-kappaB. Int Immunol 2005;17:1473–1481.
  • 28
    Spoelstra FM, Postma DS, Hovenga H, Noordhoek JA, Kauffman HF. Additive anti-inflammatory effect of formoterol and budesonide on human lung fibroblasts. Thorax 2002;57:237–241.
  • 29
    Hoshino M, Nakamura Y, Sim JJ, Yamashiro Y, Uchida K, Hosaka K et al. Inhaled corticosteroid reduced lamina reticularis of the basement membrane by modulation of insulin-like growth factor (IGF)-I expression in bronchial asthma. Clin Exp Allergy 1998;28:568–577.
    Direct Link:
  • 30
    Todorova L, Gurcan E, Miller-Larsson A, Westergren-Thorsson G. Lung fibroblast proteoglycan production induced by serum is inhibited by budesonide and formoterol. Am J Respir Cell Mol Biol 2006;34:92–100.
  • 31
    Eickelberg O, Roth M, Lorx R, Bruce V, Rudiger J, Johnson M et al. Ligand-independent activation of the glucocorticoid receptor by beta2-adrenergic receptor agonists in primary human lung fibroblasts and vascular smooth muscle cells. J Biol Chem 1999;274:1005–1010.
  • 32
    Hagood JS, Prabhakaran P, Kumbla P, Salazar L, MacEwen MW, Barker TH et al. Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis. Am J Pathol 2005;167:365–379.
  • 33
    Chung KF, Barnes PJ. Cytokines in asthma. Thorax 1999;54:825–857.
  • 34
    Romberger DJ et al. Modulation of fibronectin production of bovine bronchial epithelial cells by transforming growth factor-beta. Am J Respir Cell Mol Biol 1992;7:149–155.
View Full Article (HTML) Get PDF (191K)