• 1
    Thomas WR, Smith WA, Hales BJ, Mills KL, O’Brien RM. Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol 2002;129:118.
  • 2
    Chapman MD, Wunschmann S, Pomes A. Proteases as Th2 adjuvants. Curr Allergy Asthma Rep 2007;7:363367.
  • 3
    Kikuchi Y, Takai T, Kuhara T, Ota M, Kato T, Hatanaka H et al. Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses. J Immunol 2006;177:16091617.
  • 4
    Reese TA, Liang HE, Tager AM, Luster AD, Van Rooijen N, Voehringer D et al. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 2007;447:9296.
  • 5
    Traidl-Hoffmann C, Mariani V, Hochrein H, Karg K, Wagner H, Ring J et al. Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization. J Exp Med 2005;201:627636.
  • 6
    Boldogh I, Bacsi A, Choudhury BK, Dharajiya N, Alam R, Hazra TK et al. ROS generated by pollen NADPH oxidase provide a signal that augments antigen-induced allergic airway inflammation. J Clin Invest 2005;115:21692179.
  • 7
    Gough L, Schulz O, Sewell HF, Shakib F. The cysteine protease activity of the major dust mite allergen Der p 1 selectively enhances the immunoglobulin E antibody response. J Exp Med 1999; 190:18971902.
  • 8
    Wan H, Winton HL, Soeller C, Tovey ER, Gruenert DC, Thompson PJ et al. Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 1999;104:123133.
  • 9
    Nakamura T, Hirasawa Y, Takai T, Mitsuishi K, Okuda M, Kato T et al. Reduction of skin barrier function by proteolytic activity of a recombinant house dust mite major allergen Der f 1. J Invest Dermatol 2006;126:27192723.
  • 10
    Shakib F, Schulz O, Sewell H. A mite subversive: cleavage of CD23 and CD25 by Der p 1 enhances allergenicity. Immunol Today 1998;19:313316.
  • 11
    King C, Brennan S, Thompson PJ, Stewart GA. Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium. J Immunol 1998;161:36453651.
  • 12
    Wan H, Winton HL, Soeller C, Taylor GW, Gruenert DC, Thompson PJ et al. The transmembrane protein occludin of epithelial tight junctions is a functional target for serine peptidases from faecal pellets of Dermatophagoides pteronyssinus. Clin Exp Allergy 2001;31:279294.
  • 13
    Sun G, Stacey MA, Schmidt M, Mori L, Mattoli S. Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells. J Immunol 2001;167:10141021.
  • 14
    Seto T, Takai T, Ebihara N, Matsuoka H, Wang XL, Ishii A et al. SLPI prevents cytokine release in mite protease-exposed conjunctival epithelial cells. Biochem Biophys Res Commun 2009;379:681685.
  • 15
    Brown A, Farmer K, MacDonald L, Kalsheker N, Pritchard D, Haslett C et al. House dust mite Der p 1 downregulates defenses of the lung by inactivating elastase inhibitors. Am J Respir Cell Mol Biol 2003;29:381389.
  • 16
    Hammad H, Lambrecht BN. Dendritic cells and epithelial cells: linking innate and adaptive immunity in asthma. Nat Rev Immunol 2008;8:193204.
  • 17
    Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H et al. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol 2009;123:179186.
  • 18
    Tan BB, Weald D, Strickland I, Friedmann PS. Double-blind controlled trial of effect of housedust-mite allergen avoidance on atopic dermatitis. Lancet 1996;347:1518.
  • 19
    Yasueda H, Saito A, Nishioka K, Kutsuwada K, Akiyama K. Measurement of Dermatophagoides mite allergens on bedding and human skin surfaces. Clin Exp Allergy 2003;33:16541658.
  • 20
    Kato T, Takai T, Mitsuishi K, Okumura K, Ogawa H. Cystatin A inhibits IL-8 production by keratinocytes stimulated with Der p 1 and Der f 1: biochemical skin barrier against mite cysteine proteases. J Allergy Clin Immunol 2005;116:169176.
  • 21
    Ogawa T, Takai T, Kato T, Kikuchi T, Niyonsaba F, Ikeda S et al. Upregulation of release of granulocyte-macrophage colony-stimulating factor from keratinocytes stimulated with cysteine protease activity of recombinant major mite allergens, Der f 1 and Der p 1. Int Arch Allergy Immunol 2008;146:2735.
  • 22
    Mascia F, Mariani V, Giannetti A, Girolomoni G, Pastore S. House dust mite allergen exerts no direct proinflammatory effects on human keratinocytes. J Allergy Clin Immunol 2002;109:532538.
  • 23
    Matsuoka H, Maki N, Yoshida S, Arai M, Wang J, Oikawa Y et al. A mouse model of the atopic eczema/dermatitis syndrome by repeated application of a crude extract of house-dust mite Dermatophagoides farinae. Allergy 2003;58:139145.
  • 24
    Takai T, Kato T, Yasueda H, Okumura K, Ogawa H. Analysis of the structure and allergenicity of recombinant pro- and mature Der p 1 and Der f 1: major conformational IgE epitopes blocked by prodomains. J Allergy Clin Immunol 2005;115:555563.
  • 25
    Takahashi K, Takai T, Yasuhara T, Yokota T, Okumura Y. Effects of site-directed mutagenesis in the cysteine residues and the N-glycosylation motif in recombinant Der f 1 on secretion and protease activity. Int Arch Allergy Immunol 2001;124:454460.
  • 26
    Takai T, Kato T, Sakata Y, Yasueda H, Izuhara K, Okumura K et al. Recombinant Der p 1 and Der f 1 exhibit cysteine protease activity but no serine protease activity. Biochem Biophys Res Commun 2005;328:944952.
  • 27
    Schechter NM, Brass LF, Lavker RM, Jensen PJ. Reaction of mast cell proteases tryptase and chymase with protease activated receptors (PARs) on keratinocytes and fibroblasts. J Cell Physiol 1998;176:365373.
  • 28
    Ino Y, Ando T, Haida M, Nakamura K, Iwaki M, Okudaira H et al. Characterization of the proteases in the crude mite extract. Int Arch Allergy Appl Immunol 1989;89:321326.
  • 29
    Ando T, Ino Y, Haida M, Honma R, Maeda H, Yamakawa H et al. Isolation of cysteine protease in the crude mite extract, Dermatophagoides farinae. Int Arch Allergy Appl Immunol 1991;96:199205.
  • 30
    Ando T, Homma R, Ino Y, Ito G, Miyahara A, Yanagihara T et al. Trypsin-like protease of mites: purification and characterization of trypsin-like protease from mite faecal extract Dermatophagoides farinae. Relationship between trypsin-like protease and Der f III. Clin Exp Allergy 1993;23:777784.
  • 31
    Stewart GA, Kollinger MR, King CM, Thompson PJ. A comparative study of three serine proteases from Dermatophagoides pteronyssinus and D. farinae. Allergy 1994;49:553560.
  • 32
    Tovey ER, Chapman MD, Platts-Mills TA. Mite faeces are a major source of house dust allergens. Nature 1981;289:592593.
  • 33
    Shpacovitch V, Feld M, Bunnett NW, Steinhoff M. Protease-activated receptors: novel PARtners in innate immunity. Trends Immunol 2007;28:541550.
  • 34
    Adam E, Hansen KK, Astudillo Fernandez O, Coulon L, Bex F, Duhant X et al. The house dust mite allergen Der p 1, unlike Der p 3, stimulates the expression of interleukin-8 in human airway epithelial cells via a proteinase-activated receptor-2-independent mechanism. J Biol Chem 2006;281:69106923.
  • 35
    Ogawa H, Yoshiike T. A speculative view of atopic dermatitis: barrier dysfunction in pathogenesis. J Dermatol Sci 1993;5:197204.
  • 36
    Cork MJ, Robinson DA, Vasilopoulos Y, Ferguson A, Moustafa M, MacGowan A et al. New perspectives on epidermal barrier dysfunction in atopic dermatitis: gene-environment interactions. J Allergy Clin Immunol 2006;118:321.
  • 37
    Palmer CN, Irvine AD, Terron-Kwiatkowski A, Zhao Y, Liao H, Lee SP et al. Common loss-of-function variants of the epidermal barrier protein filaggrin are a major predisposing factor for atopic dermatitis. Nat Genet 2006;38:441446.
  • 38
    Walley AJ, Chavanas S, Moffatt MF, Esnouf RM, Ubhi B, Lawrence R et al. Gene polymorphism in Netherton and common atopic disease. Nat Genet 2001;29:175178.
  • 39
    Vasilopoulos Y, Cork MJ, Teare D, Marinou I, Ward SJ, Duff GW et al. A nonsynonymous substitution of cystatin A, a cysteine protease inhibitor of house dust mite protease, leads to decreased mRNA stability and shows a significant association with atopic dermatitis. Allergy 2007;62:514519.
  • 40
    Okuda M, Yoshiike T, Ogawa H. Detergent-induced epidermal barrier dysfunction and its prevention. J Dermatol Sci 2002;30:173179.
  • 41
    Jeong SK, Kim HJ, Youm JK, Ahn SK, Choi EH, Sohn MH et al. Mite and cockroach allergens activate protease-activated receptor 2 and delay epidermal permeability barrier recovery. J Invest Dermatol 2008;128:19301939.
  • 42
    Machado DC, Horton D, Harrop R, Peachell PT, Helm BA. Potential allergens stimulate the release of mediators of the allergic response from cells of mast cell lineage in the absence of sensitization with antigen-specific IgE. Eur J Immunol 1996;26:29722980.
  • 43
    Miike S, Kita H. Human eosinophils are activated by cysteine proteases and release inflammatory mediators. J Allergy Clin Immunol 2003;111:704713.
  • 44
    Fujisawa T, Katsumata H, Kato Y. House dust mite extract induces interleukin-9 expression in human eosinophils. Allergol Int 2008;57:141146.
  • 45
    Sokol CL, Barton GM, Farr AG, Medzhitov R. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol 2008;9:310318.