Decreased FOXP3 protein expression in patients with asthma
Article first published online: 14 APR 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Munksgaard
Volume 64, Issue 10, pages 1539–1546, October 2009
How to Cite
Provoost, S., Maes, T., Van Durme, Y. M., Gevaert, P., Bachert, C., Schmidt-Weber, C. B., Brusselle, G. G., Joos, G. F. and Tournoy, K. G. (2009), Decreased FOXP3 protein expression in patients with asthma. Allergy, 64: 1539–1546. doi: 10.1111/j.1398-9995.2009.02056.x
- Issue published online: 15 SEP 2009
- Article first published online: 14 APR 2009
- Accepted for publication 29 October 2008
- inhaled corticosteroid treatment;
- T-regulatory cells
Background: T-regulatory cells (Treg) are important in balancing immune responses and maintaining peripheral tolerance. Current evidence suggests that asthma is characterized by a relative deficiency in Treg, allowing T helper 2 cells to expand. In this study, we aimed to evaluate circulating Treg, defined by the protein FOXP3, in both control subjects and patients with stable asthma.
Methods: Peripheral blood mononuclear cells (PBMC) of control (n = 14) and asthmatic patients (n = 29) were labeled for CD4, CD25, and intracellular FOXP3 and analyzed using flow cytometry. In CD3/CD28 stimulated PBMC, the effects of dexamethasone on the transcription factors T-bet, GATA-3, FOXP3, and RORc2 and representative cytokines were studied.
Results: In control subjects and asthmatic patients, numbers of peripheral blood CD4+CD25high and CD4+CD25highFOXP3+ T-cells were similar. However, FOXP3 protein expression within CD4+CD25high T-cells was significantly decreased in asthmatic patients. There was a tendency for increased FOXP3 expression within CD4+CD25high T-cells in glucocorticosteroid-treated patients when compared to steroid-naive asthmatic patients. In stimulated PBMC, dexamethasone treatment increased the anti-/proinflammatory transcription ratios of FOXP3/GATA-3, FOXP3/T-bet, and FOXP3/RORc2.
Conclusion: Asthmatic patients have decreased FOXP3 protein expression within their CD4+CD25high Treg. Our findings also suggest that treatment with inhaled glucocorticosteroids in asthmatics might increase this FOXP3 protein expression within the CD4+CD25high T-cell population.