The allergen Bet v 1 in fractions of ambient air deviates from birch pollen counts

Authors

  • J. T. M. Buters,

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • I. Weichenmeier,

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • S. Ochs,

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • G. Pusch,

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • W. Kreyling,

    1. Institute for Inhalation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany
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  • A. J. F. Boere,

    1. National Institute for Public Health and the Environment, Centre for Environmental Health Research, Department of Toxic Effects of Air Pollution, Bilthoven, The Netherlands
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  • W. Schober,

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • H. Behrendt

    1. Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM – Center for Allergy and Environment, Technische Universität München, Munich, Germany
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  • Edited by: Wytske Fokkens

Prof. Dr J. T. M. Buters,
ZAUM – Center for Allergy and Environment, Technische Universität München, Biedersteiner Str. 29, 80802 Munich, Germany.
Tel.: +49 89 4140 3487
Fax: +49 89 4140 3453
E-mail: buters@lrz.tum.de

Abstract

To cite this article: Buters JTM, Weichenmeier I, Ochs S, Pusch G, Kreyling W, Boere AJF, Schober W, Behrendt H. The allergen Bet v 1 in fractions of ambient air deviates from birch pollen counts. Allergy 2010; 65: 850–858.

Abstract

Background:  Proof is lacking that pollen count is representative for allergen exposure, also because allergens were found in nonpollen-bearing fractions of ambient air.

Objective:  We monitored simultaneously birch pollen and the major birch pollen allergen Bet v 1 in different size fractions of ambient air from 2004 till 2007 in Munich, Germany.

Methods:  Air was sampled with a ChemVol® high-volume cascade impactor equipped with stages for particulate matter (PM)>10 μm, 10 μm>PM>2.5 μm, and 2.5 μm>PM>0.12 μm. Allergen was determined with a Bet v 1-specific ELISA. Pollen count was assessed with a Burkard pollen trap. We also measured the development of allergen in pollen during ripening.

Results:  About 93 ± 3% of Bet v 1 was found in the PM > 10 μm fraction, the fraction containing birch pollen. We did not measure any Bet v 1 in 2.5 μm > PM > 0.12 μm. Either in Munich no allergen was in this fraction or the allergen was absorbed to diesel soot particles that also deposit in this fraction. Pollen released 115% more Bet v 1 in 2007 than in 2004. Also within 1 year, the release of allergen from the same amount of pollen varied more than 10-fold between different days. This difference was explained by a rapidly increasing expression of Bet v 1 in pollen in the week just before pollination. Depending on the day the pollen is released during ripening, its potency varies.

Conclusion:  In general, pollen count and allergen in ambient air follow the same temporal trends. However, because a 10-fold difference can exist in allergen potency of birch pollen, symptoms might be difficult to correlate with pollen counts, but perhaps better with allergen exposure.

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