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Data S1. Additional information on Subjects and Methods, Statistical Analysis, and Results (including Sex-Specific Association Analysis for Total IgE [Table S2] and Immunohistochemistry).

Figure S1. Human endobronchial biopsy samples from asthmatic patients were fixed, embedded, and serially sectioned. Paraffin-embedded tissues were hydrated, retrieved, and immunostained with anti-TSLP (in dark brown). Images were taken with a conventional light microscope using 200x magnification.

Figure S2. The upper portion of the figure presents the sex and age specific current asthma prevalence rates in the United States from the Centers of Disease Control, National Health Interview Survey, 2006 (http://www.cdc.gov/asthma/nhis/06/table 4-1.htm). The bottom portion of the figure presents the age-specific interquartile ranges of subjects from Costa Rica (in red), Childhood Asthma Management Program (CAMP-in light green for white subjects, in dark green for African-American subjects), the Children’s Health Study (CHS-in pink), the Genome Research on the African-American Diaspora (GRAAD-in light blue), and in the FraminghamHeart Study (FHS-in yellow).

Table S1. Minor Allele Frequencies of Two TSLP SNP in the Costa Rica, Childhood Asthma Management Program (CAMP), Children’s Health (CHS), the Genomic Research on Asthma in the African Diaspora (GRAAD), and the Framingham Heart Studies (FHS) and the HapMap CEPH and Yoruban Poulations.

Table S2. Association between TSLP SNP and Total IgE in the Costa Rica, Childhood Asthma Management Program (CAMP), and the Genomic Research on Asthma in the African Diaspora (GRAAD) studies.

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