Edited by: Hans-Uwe Simon
Impact of systemic immuno-suppression after solid organ transplantation on allergen-specific responses
Article first published online: 7 SEP 2010
© 2010 John Wiley & Sons A/S
Volume 66, Issue 2, pages 271–278, February 2011
How to Cite
Eiwegger, T., Gruber, S., Geiger, C., Mayer, E., Dehlink, E., Bannert, C., Frischer, T., Kasper, D., Jaksch, P., Klepetko, W., Akdis, C. and Szépfalusi, Z. (2011), Impact of systemic immuno-suppression after solid organ transplantation on allergen-specific responses. Allergy, 66: 271–278. doi: 10.1111/j.1398-9995.2010.02475.x
- Issue published online: 5 JAN 2011
- Article first published online: 7 SEP 2010
- Accepted for publication 22 July 2010
- cyclosporin A;
- regulatory T-cells;
To cite this article: Eiwegger T, Gruber S, Geiger C, Mayer E, Dehlink E, Bannert C, Frischer T, Kasper D, Jaksch P, Klepetko W, Akdis C, Szépfalusi Z. Impact of systemic immuno-suppression after solid organ transplantation on allergen-specific responses. Allergy 2011; 66: 271–278.
Introduction: The immunosuppressive therapy in solid organ transplantation targets mainly the T- and B-cell-mediated immune response. However, there is evidence that it neither suppresses sensitization nor clinical manifestation of allergic diseases in organ-transplanted patients.
Objective: This study addresses the question whether allergen-specific responses are altered by systemic immunosuppression via negative effects on the T-regulatory cell compartment and a more pronounced suppression on Th1-type T-cell responses.
Material and methods: Peripheral blood mononuclear cells from 65 solid organ-transplanted (kidney, liver, lung) children, adolescents, and young adults and 18 healthy, matched controls were included, and their clinical and sensitization status assessed. Allergen-specific proliferation, intracellular cytokine production, frequency of forkhead box P3 (FOXP3)+CD3+CD4+CD25high cells, mRNA expression of IL-10, transforming growth factor (TGF)-β and FOXP3 (real-time RT-PCR) of peripheral blood mononuclear cells or bronchoalveolar lavage fluid (BAL)-derived cells, and the inhibitory capacity of T-reg cells were investigated.
Results: Immunosuppression led to a significantly altered regulatory marker profile expressed by enhanced TGF-β mRNA production and a reduced frequency of FOXP3+CD4+CD3+ cells in solid organ transplanted individuals. FOXP3 expression in BAL cells of lung-transplanted patients was significantly decreased. Allergen-specific proliferation was not significantly altered despite long-term immunosuppression. However, suppression of allergen-specific responses via the T-regulatory cell fraction was deficient in immunosuppressed individuals.
Conclusion: The results suggest an insufficient control of allergen-specific responses via the Treg-cell compartment under systemic immunosuppression.