Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization

Authors

  • M. Schwarzer,

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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    • Both authors contributed equally to the work.

  • A. Repa,

    1. Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna
    2. Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria
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    • Both authors contributed equally to the work.

  • C. Daniel,

    1. Institut Pasteur de Lille, Lactic acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Univ Lille Nord de France, CNRS, UMR 8204, Institut National de la Santé et de la Recherche Médicale, U1019, Lille, France
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  • I. Schabussova,

    1. Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna
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  • T. Hrncir,

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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  • B. Pot,

    1. Institut Pasteur de Lille, Lactic acid Bacteria & Mucosal Immunity, Center for Infection and Immunity of Lille, Univ Lille Nord de France, CNRS, UMR 8204, Institut National de la Santé et de la Recherche Médicale, U1019, Lille, France
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  • R. Stepankova,

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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  • T. Hudcovic,

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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  • A. Pollak,

    1. Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Vienna, Austria
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  • H. Tlaskalova-Hogenova,

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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  • U. Wiedermann,

    1. Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna
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  • H. Kozakova

    1. Department of Immunology and Gnotobiology, Institute of Microbiology of the Academy of Sciences of the Czech Republic, v. v. i., Novy Hradek, Czech Republic
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  • Edited by: Hans-Uwe Simon

Univ. Prof. Dr. Ursula Wiedermann, Department of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Tel.: +431-4049-064890
Fax: +431-4049-064899
E-mail: ursula.wiedermann@meduniwien.ac.at

Abstract

To cite this article: Schwarzer M, Repa A, Daniel C, Schabussova I, Hrncir T, Pot B, Stepankova R, Hudcovic T, Pollak A, Tlaskalova-Hogenova H, Wiedermann U, Kozakova H. Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization. Allergy 2011; 66: 368–375.

Abstract

Background:  The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen-defined allergy prevention/treatment. Interventions with LAB are of increasing interest early in life when immune programming is initiated. Here, we investigated the effect of neonatal colonization with a recombinant LAB producing the major birch pollen allergen Bet v 1 in a murine model of type I allergy.

Methods:  We constructed a recombinant Lactobacillus (L.) plantarum NCIMB8826 strain constitutively producing Bet v 1 to be used for natural mother-to-offspring mono-colonization of germ-free BALB/c mice. Allergen-specific immunomodulatory effects of the colonization on humoral and cellular immune responses were investigated prior and after sensitization to Bet v 1.

Results:  Mono-colonization with the Bet v 1 producing L. plantarum induced a Th1-biased immune response at the cellular level, evident in IFN-γ production of splenocytes upon stimulation with Bet v 1. After sensitization with Bet v 1 these mice displayed suppressed IL-4 and IL-5 production in spleen and mesenteric lymph node cell cultures as well as decreased allergen-specific antibody responses (IgG1, IgG2a, and IgE) in sera. This suppression was associated with a significant up-regulation of the regulatory marker Foxp3 at the mRNA level in the spleen cells.

Conclusion:  Intervention at birth with a live recombinant L. plantarum producing a clinically relevant allergen reduces experimental allergy and might therefore become an effective strategy for early intervention against the onset of allergic diseases.

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