Edited by: Hans-Uwe Simon
Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus
Article first published online: 29 OCT 2010
© 2010 John Wiley & Sons A/S
Volume 66, Issue 3, pages 386–395, March 2011
How to Cite
Bratke, K., Klein, C., Kuepper, M., Lommatzsch, M. and Virchow, J. C. (2011), Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. Allergy, 66: 386–395. doi: 10.1111/j.1398-9995.2010.02497.x
- Issue published online: 1 FEB 2011
- Article first published online: 29 OCT 2010
- Accepted for publication 23 September 2010
- blood dendritic cell antigen-3 (BDCA-3);
- interleukin-4 (IL-4);
- plasmacytoid dendritic cell development;
- T cell polarization;
- T cell proliferation
To cite this article: Bratke K, Klein C, Kuepper M, Lommatzsch M, Christian Virchow J. Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. Allergy 2011; 66: 386–395.
Background: Plasmacytoid dendritic cells (pDCs) infiltrate sites of Th1- and Th2-dominant inflammation and many studies have been performed to analyse their role in these immune responses. In contrast, much less is known about the effects of a Th1 or Th2 cytokine milieu on pDC function. Therefore, we investigated the impact of Th1- and Th2-like conditions during the development of pDCs on their antigen expression and function.
Methods: PDCs were matured in vitro by the addition of IL-3 under Th1- or Th2-like conditions. Antigen expression and TLR7-ligand-induced cytokine secretion was analysed by flow cytometry and ELISA. Furthermore, the CD4+ T-cell polarizing capacity of pDCs was determined as well as their potential to induce CD4+ T-cell proliferation.
Results: PDCs matured under Th1-like conditions showed a higher expression of antigens involved in T-cell co-stimulation and antigen presentation like CD40, CD80, CD83 and HLA-DR as well as a higher secretion of IL-6 and IFN-α in response to TLR7-ligation compared to Th2-pDCs. Furthermore, Th1-pDCs induced a significantly higher CD4+ T-cell proliferation and primed a higher percentage of CD4+ T cells to express IFN-γ and IL-2 after TLR7-ligation compared to Th2-pDCs. In contrast, Th2-pDCs were characterized by a significant upregulation of BDCA-3 and IL-4 expression following TLR7-ligation.
Conclusion: This study is the first to demonstrate the crucial impact of a surrounding cytokine environment on the development of pDC function including antigen expression. Based on these findings, it can be speculated that antiviral/bacterial pDC functions could be impaired during acute allergic conditions.