These authors contribute equally to this paper.
A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies
Article first published online: 18 AUG 2011
© 2011 John Wiley & Sons A/S
Volume 66, Issue 11, pages 1420–1427, November 2011
How to Cite
An, S., Ma, D., Wei, J. F., Yang, X., Yang, H. W., Yang, H., Xu, X., He, S. and Lai, R. (2011), A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies. Allergy, 66: 1420–1427. doi: 10.1111/j.1398-9995.2011.02683.x
The IUIS Allergen Nomenclature Sub-Committee (http://www.allergen.org) has assigned to the purified allergen (apyrase) the official number Tab y 1.0101, and the suggested allergenicity results are listed under Tab y 1.
Edited by: Reto Crameri.
- Issue published online: 7 OCT 2011
- Article first published online: 18 AUG 2011
- Accepted for publication 9 July 2011
- horsefly allergen;
- insect allergy
To cite this article: An S, Ma D, Wei JF, Yang X, Yang HW, Yang H, Xu X, He S, Lai R. A novel allergen Tab y 1 with inhibitory activity of platelet aggregation from salivary glands of horseflies. Allergy 2011; 66: 1420–1427.
Background: Horsefly sting causes allergic reactions in human body. However, our knowledge on horsefly allergens remains poor.
Objectives: To identify the novel horsefly allergens and characterize their properties.
Methods: A native allergen protein Tab y 1 (apyrase) was purified from the salivary glands of the horsefly Tabanus yao Macquart by gel filtration and ion exchange chromatography. Its sequence was determined by Edman degradation and cDNA cloning. Its allergenicity was assessed by immunoblotting for specific IgE, basophil activation test, skin prick test (SPT), and competitive enzyme-linked immunosorbent assay (ELISA).
Results: Tab y 1 showed a single diffusion band of 70 kDa on SDS-PAGE. Seventy percent (7/10) of patients with horsefly allergy tested positive to Tab y 1 in SPT; sera from 81% (30/37) of patients reacted to Tab y 1 on western blots. Purified Tab y 1 reduced approximately 42% sera IgE reactivity to horsefly salivary gland extract on a competitive ELISA. Tab y 1 upregulated the expression of CD63 and CCR3 on passively sensitized basophils by up to approximately 4.9-fold. Tab y 1 also showed enzymatic activity to hydrolyze ATP and ADP, and potent antiplatelet aggregation and antithrombotic activities.
Conclusion: The current work identified a novel major allergen of horsefly, Tab y 1, with antiplatelet aggregation and antithrombotic activities, which implicates Tab y 1 in helping horseflies suck host blood, meanwhile causing allergy in their human hosts.