Cross-reactions vs co-sensitization evaluated by in silico motifs and in vitro IgE microarray testing

Authors


Beda M. Stadler, University Institute of Immunology, Sahli Haus 2, Inselspital, 3010 Bern, Switzerland.
Tel.: +41 (0)31 632 35 21
Fax: +41 (0)31 381 57 35
E-mail: beda.stadler@iib.unibe.ch

Abstract

To cite this article: Pfiffner P, Stadler BM, Rasi C, Scala E, Mari A. Cross-reactions vs co-sensitization evaluated by in silico motifs and in vitro IgE microarray testing. Allergy 2012; 67: 210–216.

Abstract

Background and objective:  Using an in silico allergen clustering method, we have recently shown that allergen extracts are highly cross-reactive. Here we used serological data from a multi-array IgE test based on recombinant or highly purified natural allergens to evaluate whether co-reactions are true cross-reactions or co-sensitizations by allergens with the same motifs.

Methods:  The serum database consisted of 3142 samples, each tested against 103 highly purified natural or recombinant allergens. Cross-reactivity was predicted by an iterative motif-finding algorithm through sequence motifs identified in 2708 known allergens.

Results:  Allergen proteins containing the same motifs cross-reacted as predicted. However, proteins with identical motifs revealed a hierarchy in the degree of cross-reaction: The more frequent an allergen was positive in the allergic population, the less frequently it was cross-reacting and vice versa. Co-sensitization was analyzed by splitting the dataset into patient groups that were most likely sensitized through geographical occurrence of allergens. Interestingly, most co-reactions are cross-reactions but not co-sensitizations.

Conclusions:  The observed hierarchy of cross-reactivity may play an important role for the future management of allergic diseases.

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