Edited by: Thomas Bieber
Basophils are not the key antigen-presenting cells in allergic patients
Article first published online: 16 FEB 2012
© 2012 John Wiley & Sons A/S
Volume 67, Issue 5, pages 601–608, May 2012
How to Cite
Eckl-Dorna J, Ellinger A, Blatt K, Ghanim V, Steiner I, Pavelka M, Valent P, Valenta R, Niederberger V. Basophils are not the key antigen-presenting cells in allergic patients. Allergy 2012; 67: 601–608.
- Issue published online: 11 APR 2012
- Article first published online: 16 FEB 2012
- Manuscript Accepted: 3 JAN 2012
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Recent data obtained in mouse models have initiated a controversy whether basophils are the key antigen-presenting cells (APCs) in allergy. Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients.
T cells, basophils, and APCs depleted of basophils were purified from allergic patients. Co-culture systems based on purified major allergens were established to study allergen-specific T cell responses using proliferation assays.
Only co-cultures of T cells with APCs depleted of basophils but not with basophils proliferated in response to allergen. Even addition of IL-3 to T cell–basophil co-cultures failed to induce allergen-specific T cell proliferation.
Our data demonstrate by classical in vitro proliferation assays that basophils are not key antigen-presenting cells that promote T cell proliferation in secondary immune responses to allergen in allergic patients.