Edited by: Sarbjit Saini
Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients
Article first published online: 17 FEB 2012
© 2012 John Wiley & Sons A/S
Volume 67, Issue 5, pages 609–621, May 2012
How to Cite
Chen K-W, Focke-Tejkl M, Blatt K, Kneidinger M, Gieras A, Dall'Antonia F, Faé I, Fischer G, Keller W, Valent P, Valenta R, Vrtala S. Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients. Allergy 2012; 67: 609–621.
- Issue published online: 11 APR 2012
- Article first published online: 17 FEB 2012
- Manuscript Accepted: 5 JAN 2012
- Austrian Science Fund
- Christian Doppler Research Association
- Der p 2;
- house dust mite allergy;
More than 90% of house dust mite-allergic patients are sensitized to the major Dermatophagoides pteronyssinus allergen, Der p 2. The aim of this study was to develop and characterize an allergy vaccine based on carrier-bound Der p 2 peptides, which should allow reducing IgE- and T-cell-mediated side-effects during specific immunotherapy (SIT).
Five Der p 2 peptides (P1–P5) were synthesized and analyzed regarding IgE reactivity and allergenic activity. Lymphoproliferative and cytokine responses induced with Der p 2 and Der p 2 peptides were determined in peripheral blood mononuclear cells from mite-allergic patients. Der p 2-specific IgG antibodies induced with carrier-bound Der p 2 peptides in mice and rabbits were tested for their capacity to inhibit IgE binding and basophil activation in allergic patients.
Of five overlapping peptides (P1–P5) covering the Der p 2 sequence, two peptides (P2 and P4) were identified, which showed no relevant IgE reactivity, allergenic activity, and induced lower Der p 2-specific T-cell activation than Der p 2. However, when coupled to a carrier, P2 and P4 induced Der p 2-specific IgG antibodies in animals, which inhibited allergic patients' IgE binding to the allergen and allergen-induced basophil activation similar as antibodies induced with Der p 2.
Carrier-bound Der p 2 peptides should allow avoiding IgE-mediated side-effects, and because of their low potential to activate allergen-specific T cells, they may reduce late-phase side-effects during SIT. Further, these peptides may be also useful for prophylactic vaccination.