Both authors contributed equally to this work.
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Original Article
Nonirritant intradermal skin test concentrations of ciprofloxacin, clarithromycin, and rifampicin
Article first published online: 22 MAR 2012
DOI: 10.1111/j.1398-9995.2012.02807.x
© 2012 John Wiley & Sons A/S
Additional Information
How to Cite
Brož P, Harr Th, Hecking C, Grize L, Scherer K, Jaeger KA, Bircher AJ. Nonirritant intradermal skin test concentrations of ciprofloxacin, clarithromycin, and rifampicin. Allergy 2012; 67: 647–652.
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Both authors contributed equally to this work.
Edited by: Werner Aberer
Publication History
- Issue published online: 11 APR 2012
- Article first published online: 22 MAR 2012
- Manuscript Accepted: 31 JAN 2012
- Abstract
- Article
- References
- Cited By
Keywords:
- antibiotics allergy;
- ciprofloxacin;
- clarithromycin;
- digital image analysis;
- intradermal testing;
- laser Doppler flowmetry;
- rifampicin
Abstract
Background
Intradermal skin testing of the clinically important antibiotics ciprofloxacin, clarithromycin, and rifampicin in the case of suspected allergies to antibiotics is poorly standardized. For clinical practice, standardized procedures and protocols are desired.
Methods
Fifteen healthy volunteers were tested with different concentrations of the antibiotics as well as with appropriate controls. Test readings included wheal area measured by digital image analysis and blood flow increase measured by laser Doppler flowmetry (LDF). To reduce interpersonal variability, test results were normalized with the individual controls using a novel protocol.
Results
Nonirritating concentrations of the three antibiotics (ciprofloxacin ~0.0067 mg/ml, clarithromycin ~0.05 mg/ml, rifampicin ~0.002 mg/ml) could be defined for healthy volunteers. Laser Doppler flowmetry generates comparable results to wheal area measurement. Normalization of the test results is necessary and can be applied in a practical algorithm.
Conclusions
Standardized skin testing to detect sensitization to broadly used nonbetalactam antibiotics was presented and should be applied in truly sensitized patients. This approach should help to minimize the inter- and intraindividual differences in reactivity.