Edited by: Reto Crameri
Characterization of the dog lipocalin allergen Can f 6: the role in cross-reactivity with cat and horse
Article first published online: 20 APR 2012
© 2012 John Wiley & Sons A/S
Volume 67, Issue 6, pages 751–757, June 2012
How to Cite
Nilsson OB, Binnmyr J, Zoltowska A, Saarne T, van Hage M, Grönlund H. Characterization of the dog lipocalin allergen Can f 6: the role in cross-reactivity with cat and horse. Allergy 2012; 67: 751–757.
- Issue published online: 10 MAY 2012
- Article first published online: 20 APR 2012
- Manuscript Accepted: 13 MAR 2012
- Swedish Asthma and Allergy Association's Research Foundation
- Theme Center for Inflammatory Diseases and Karolinska Institutet
- Konsul Th C Bergh Foundation
- Cancer and Allergy Foundation
- Swedish Research Council
- Swedish Heart-Lung Foundation
- King Gustaf V 80th Birthday Foundation
- Hesselman Foundation
- Centre for Allergy Research and the Bernard Osher Initiative for Research on Severe Asthma at Karolinska Institutet
- Stockholm County Council
- Can f 6;
- dog allergy;
- dog dander
Allergy to the domestic dog (Canis familiaris) affects 5–10% of the population in affluent countries. Three of four patients are allergic to more than one pet, which can only partially be explained by cross-reactivity between serum albumins. The lipocalin protein family harbours allergens in mammalian species.
We set out to clone and characterize a novel dog allergen, and investigate its potential role in cross-sensitization between dog, cat and horse. The gene encoding Can f 6 was amplified from dog skin and bladder cDNA libraries. The corresponding allergen was produced and purified by recombinant techniques and evaluated by SDS-PAGE, size exclusion chromatography, circular dichroism spectra, ELISA and basophil activation test.
IgE antibodies to Can f 6 were found in serum from 38% of dog-sensitized subjects. Sequence similarities between the lipocalin allergens Can f 6, Fel d 4 (cat) and Equ c 1 (horse) suggested a probability for cross-reactivity, which was demonstrated by competitive ELISA. The biological relevance of Can f 6 was confirmed by basophil activation test in dog-allergic patients.
Can f 6 is a new lipocalin dog allergen that cross-reacts with lipocalins from horse and cat. Can f 6 and homologous allergens may contribute to multisensitization and symptoms in individuals allergic to mammals.