These authors contributed equally to this work.
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Original Article
Ragweed pollen collected along high-traffic roads shows a higher allergenicity than pollen sampled in vegetated areas
Article first published online: 14 MAY 2012
DOI: 10.1111/j.1398-9995.2012.02846.x
© 2012 John Wiley & Sons A/S
Additional Information
How to Cite
Ghiani A, Aina R, Asero R, Bellotto E, Citterio S. Ragweed pollen collected along high-traffic roads shows a higher allergenicity than pollen sampled in vegetated areas. Allergy 2012; DOI:10.1111/j.1398-9995.2012.02846.x.
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These authors contributed equally to this work.
Edited by: Reto Crameri
Publication History
- Issue published online: 8 JUN 2012
- Article first published online: 14 MAY 2012
- Manuscript Accepted: 14 APR 2012
Funded by
- Fondazione Banca del Monte di Lombardia
Keywords:
- Ambrosia artemisiifolia;
- ragweed pollen allergens;
- sub-pollen particles;
- traffic-related pollutants
Abstract
Background
Pollutants may affect pollen allergenicity and thus the prevalence of allergies. Although a few studies are available in literature, the connection between pollution and the allergenic potential of pollen has yet to be clearly defined. The objective of this study was to evaluate the effect of traffic-related pollution on the allergenicity of ragweed (Ambrosia artemisiifolia L.) pollen through a field-based experiment.
Methods
Mature pollen grains were collected from ragweed plants grown along main roadsides and in vegetated areas of Po river plain. The percentage of sub-pollen particle-releasing grains (SPPGs) was evaluated immediately after sampling by microscope and image analysis. Immunochemistry and LC-MS/MS were applied to assess the whole allergenicity and the allergen pattern characterizing the different pollen samples.
Results
No statistical difference was detected in the percentage of SPPGs among pollen samples. Specifically, after hydration, the mean percentage was very low (<4%) in all the samples, regardless of the site of origin. On the contrary, pollen collected along high-traffic roads showed a higher whole allergenicity than pollen from low-traffic roads and vegetated areas which showed a reactivity similar to that of the commercial pollen ‘Allergon’, used as a standard. The detected higher allergenicity levels were attributed to both quantitative and qualitative differences in allergen pattern.
Conclusion
Our findings show that pollen collected at different sites contains different amount and number of allergens and suggest that traffic-related pollution enhances ragweed pollen allergenicity, which may contribute to the increasing prevalence of ragweed allergy in Lombardy plain.