DFNB74, a novel autosomal recessive nonsyndromic hearing impairment locus on chromosome 12q14.2-q15

Authors

  • AM Waryah,

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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  • A Rehman,

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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  • ZM Ahmed,

    1. Section on Human Genetics, Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD, USA
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  • Z-H Bashir,

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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  • SY Khan,

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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  • AU Zafar,

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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  • S Riazuddin,

    Corresponding author
    1. Section on Human Genetics, Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD, USA
      Dr. Sheikh Riazuddin, National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan.
      Tel.: 92-42-5421235/1350;
      fax: 92-42-5421316;
      e-mail: riaz@lhr.comsats.net.pk
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  • TB Friedman,

    1. Section on Human Genetics, Laboratory of Molecular Genetics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Rockville, MD, USA
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  • S Riazuddin

    1. National Centre of Excellence in Molecular Biology, Punjab University, Lahore, Pakistan
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Dr. Sheikh Riazuddin, National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan.
Tel.: 92-42-5421235/1350;
fax: 92-42-5421316;
e-mail: riaz@lhr.comsats.net.pk

Abstract

Autosomal recessive nonsyndromic hearing impairment (ARNSHI) segregating in three unrelated, large consanguineous Pakistani families (PKDF528, PKDF859 and PKDF326) is linked to markers on chromosome 12q14.2-q15. This novel locus is designated DFNB74. Maximum two-point limit of detection (LOD) scores of 5.6, 5.7 and 2.6 were estimated for markers D12S313,D12S83 and D12S75 at θ = 0 for recessive deafness segregating in these three families. Haplotype analyses identified a critical linkage interval of 5.35 cM (5.36 Mb) defined by D12S329 at 74.58 cM and D12S313 at 79.93 cM. DFNB74 is the second ARNSHI locus mapped to chromosome 12, but the physical intervals do not overlap with one another. A locus contributing to the early onset, rapidly progressing hearing loss of A/J mice (ahl4, age-related hearing loss 4) was reported to map to chromosome 10 in a region of conserved synteny to DFNB74, suggesting that ahl4 and DFNB74 may be due to mutations of the same gene in these two species.

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