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Supporting Information

The following Supporting information is available for this article:

Fig. S1. Denaturing high-performance liquid chromatography (DHPLC) electropherograms. (a) Electropherograms showing c.89A[RIGHTWARDS ARROW]G, predicting p.Glu30Gly in the ZFPM2/FOG2 gene from a patient (N6) with double outlet right ventricle (DORV) vs wild type. (b) Electropherograms showing c.679A[RIGHTWARDS ARROW]G, predicting p.Ile227Val in the ZFPM2/FOG2 gene from a patient (N3) with DORV vs wild type. (c) Electropherograms showing c.1632G[RIGHTWARDS ARROW]A, predicting p.Met544Ile in the ZFPM2/FOG2 gene from a patient (N106) with tetralogy of Fallot (TOF) vs wild type.

Fig. S2. (a) DNA sequencing chromatograms showing heterozygosity in ZFPM2/FOG2 coding region. Arrows indicate the variant nucleotides. Wild-type and mutated sequences are shown below the electropherograms (mutations are boxed in red). (a) Chromatogram showing c.89A[RIGHTWARDS ARROW]G, predicting p.Glu30Gly in the ZFPM2/FOG2 gene from a patient (N6) with double outlet right ventricle (DORV). (b) Chromatogram showing c.679A[RIGHTWARDS ARROW]G, predicting p.Ile227Val in the ZFPM2/FOG2 gene from a patient (N3) with DORV. (c) Chromatogram showing c.1632G[RIGHTWARDS ARROW]A, predicting p.Met544Ile in the ZFPM2/FOG2 gene from a patient (N106) with tetralogy of Fallot (TOF).

Fig. S3. Denaturing high-performance liquid chromatography (DHPLC) electropherograms showing c.73C[RIGHTWARDS ARROW]T, predicting p.Arg25Cys in the NKX2.5 gene from patients N120 (a) and N157 (b), both affected by tetralogy of Fallot (TOF). *This patient was compound heterozygotes for the c.73C[RIGHTWARDS ARROW]T variant and the c.63A[RIGHTWARDS ARROW]G (p.Glu21Glu) polymorphism in the NKX2.5 gene.

Fig. S4. Chromatograms showing c.73C[RIGHTWARDS ARROW]T, predicting p.Arg25 Cys in the NKX2.5 gene from patients N120 (a) and N157 (b), both affected by tetralogy of Fallot (TOF). Arrows indicate the variant nucleotides. Wild-type and mutated sequences are shown below the electropherograms (mutations are boxed in red).

Table S1. Primers, polymerase chain reaction (PCR), and denaturing high-performance liquid chromatography (DHPLC) conditions for analysis of GATA4, NKX2.5, ZFPM2/FOG2, GDF1, and ISLET1 exons.

Additional Supporting information may be found in the online version of this article.

FilenameFormatSizeDescription
CGE_1523_sm_fS1.ppt1206KSupporting info item
CGE_1523_sm_fS2.ppt128KSupporting info item
CGE_1523_sm_fS3.ppt535KSupporting info item
CGE_1523_sm_fS4.ppt108KSupporting info item
CGE_1523_sm_tS1.doc70KSupporting info item

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