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Prevalence and molecular characterization of abnormal hemoglobin in eastern Guangdong of southern China

Authors

  • M Lin,

    1. Laboratory Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, P. R. China
    2. Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Chaozhou 521021, Guangdong Province, P. R. China
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  • Q Wang,

    Corresponding author
    1. Laboratory Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, P. R. China
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  • L Zheng,

    1. Laboratory Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, P. R. China
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  • Y Huang,

    1. Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Chaozhou 521021, Guangdong Province, P. R. China
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  • F Lin,

    1. Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Chaozhou 521021, Guangdong Province, P. R. China
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  • CP Lin,

    1. Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Chaozhou 521021, Guangdong Province, P. R. China
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  • LY Yang

    Corresponding author
    1. Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Chaozhou 521021, Guangdong Province, P. R. China
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Qian Wang, Laboratory Medical Center, Nanfang Hospital, Southern Medical University, No. 1838 Dadao Rd. Guangzhou 510515, Guangdong Province, P. R. China.
Tel.: +86 20 61642147;
fax: +86 20 61641023;
e-mail: wangqian@fimmu.com

Li-Ye Yang, Central Laboratory, Chaozhou Central Hospital, No. 84 Huancheng-Xi Road, Chaozhou 521021, Guangdong Province, P. R. China.
Tel.: +86 768 2255161;
fax: +86 768 2229563;
e-mail: yangleeyee@sina.com

Abstract

Lin M, Wang Q, Zheng L, Huang Y, Lin F, Lin CP, Yang LY. Prevalence and molecular characterization of abnormal hemoglobin in eastern Guangdong of southern China.

Abnormal hemoglobins (Hbs) are the most commonly inherited disorders in humans. Their frequency and types change considerably with geographic location and ethnic group. To investigate the molecular epidemiological characterization of abnormal Hbs in eastern Guangdong of southern China, a total of 11,450 ‘healthy’ subjects were subjected to hemoglobin electrophoresis screening. Samples of EDTA-K2 blood with abnormal Hbs were analyzed by CELL-DYN1700 blood analyzer; thalassemia genotypes and Hb E variant were identified by gap-PCR and/or reverse dot blot (RDB). The genotypes of Hb variants were detected by PCR and sequencing. The incidence of abnormal Hbs was 0.358%(41/11,450) in Chaozhou, including 12.2% (5/41) Hb J, 4.9% (2/41) Hb K, 9.7% (4/41) Hb Q, 31.7% (13/41) Hb G/D and 41.5% (17/41) Hb E. Eight types of Hb variants were found, including 3 cases of Hb J-Bangkok, 2 cases of Hb J-Wenchang-Wuming, 2 cases of Hb New York, 4 cases of Hb Q-Thailand, 5 cases of Hb G-Waimanalo, 4 cases of Hb Ottawa, 4 cases of Hb G-Chinese and 17 cases of Hb E. In comparison with other areas of Guangdong, Chaozhou had a different pattern of abnormal Hbs with a high prevalence of Hb G/D. This study describes the prevalence and molecular characterization of abnormal Hbs in eastern Guangdong.

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