Non-HLA T-cell reactivity during the first year after HLA-identical living-related kidney transplantation

  1. Jeroen H. Gerrits1,
  2. Jacqueline Van De Wetering1,
  3. Jos J. Drabbels3,
  4. Jan N.M. IJzermans2,
  5. Frans H.J. Claas3,
  6. Willem Weimar1,
  7. Nicole M. Van Besouw1

Article first published online: 26 JUN 2009

DOI: 10.1111/j.1399-0012.2009.01014.x

Issue

Clinical Transplantation

Clinical Transplantation

Volume 23, Issue 5, pages 740–747, September/October 2009

Additional Information

How to Cite

Gerrits, J. H., Van De Wetering, J., Drabbels, J. J., IJzermans, J. N., Claas, F. H., Weimar, W. and Van Besouw, N. M. (2009), Non-HLA T-cell reactivity during the first year after HLA-identical living-related kidney transplantation. Clinical Transplantation, 23: 740–747. doi: 10.1111/j.1399-0012.2009.01014.x

Author Information

  1. 1

    Department of Internal Medicine – Transplantation

  2. 2

    Department of Surgery, Erasmus Medical Center – University Medical Center Rotterdam, Rotterdam, The Netherlands

  3. 3

    Department of Immunohaematology and Bloodtransfusion, Leiden University Medical Center, Leiden, The Netherlands

*Corresponding author: Dr. Nicole M. van Besouw, Erasmus MC – University Medical Center Rotterdam, Department of Internal Medicine – Transplantation, Room Ee-559, PO Box 2040, NL-3000 CA Rotterdam, The Netherlands. Tel.: 31 10 703 5419; fax: 31 10 704 4718; e-mail: n.vanbesouw@erasmusmc.nl

Publication History

  1. Issue published online: 1 OCT 2009
  2. Article first published online: 26 JUN 2009
  3. Accepted for publication 13 April 2009

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Keywords:

  • Elispot;
  • kidney transplantation;
  • minor histocompatibility antigens;
  • non-HLA antigens;
  • T-cell reactivity

Abstract:  Background:  It has been reported that donor-reactive T-cell responses may decrease during the first year after HLA-mismatched organ transplantation. We wondered whether donor-reactive T-cell responses directed to minor histocompatibility antigens (mHAgs) or other non-HLA antigens also decrease after HLA-identical living-related (LR) kidney transplantation.

Methods:  We studied donor-reactive T-cell responses by IFN-γ and granzyme B (GrB) Elispot assays in 15 HLA-identical LR kidney transplant recipients before, six months and one yr after transplantation. Third-party reactivity was used as control. Patient and donor peripheral blood mononuclear cells were typed for 11 known mHAgs.

Results:  During the study period, 60% and 36% of the patients demonstrated donor-reactive IFN-γ and GrB producing cells (pc), respectively. The number of donor-reactive IFN-γ and GrB pc was significantly lower than the number of third-party reactive IFN-γ and GrB pc. After transplantation, donor-reactivity and third-party reactivity were comparable to pre-transplant values. No relation was found in mHAg mismatches between donor and recipient and donor-reactive T-cell response.

Conclusions:  Donor-reactivity could be detected before and after HLA-identical LR kidney transplantation, but was not related with the number of mHAg mismatches, and did not decrease after transplantation.

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