This study was supported by Research Grants USPHS HE-05435, RR-05425 and RR-00350 and Training Grant AI-00325 from the National Institutes of Health and by The Veterans Administration Hospital, Houston, Texas.
Detection of Alloantibodies Using a Sensitive Antiglobulin Microcytotoxicity Test: Identification of Low Levels of Pre-Formed Antibodies in Accelerated Allograft Rejection
Article first published online: 9 OCT 2008
Volume 2, Issue 4, pages 215–226, July 1972
How to Cite
JOHNSON, A. H., ROSSEN, R. D. and BUTLER, W. T. (1972), Detection of Alloantibodies Using a Sensitive Antiglobulin Microcytotoxicity Test: Identification of Low Levels of Pre-Formed Antibodies in Accelerated Allograft Rejection. Tissue Antigens, 2: 215–226. doi: 10.1111/j.1399-0039.1972.tb00138.x
- Issue published online: 9 OCT 2008
- Article first published online: 9 OCT 2008
- Received for publication 10 January, accepted 31 January 1972
An antiglobulin test, more sensitive than established direct microcytotoxicity tests, was developed to detect both complement fixing and non-complement fixing alloantibodies. The test has been used to detect antibodies in sera from selected organ allograft recipients. Of 56 sera tested by the antiglobulin method, 52 (93%) were reactive. In contrast, only 13 (23%) were reactive in an established direct microcytotoxicity test and only 28 (50%) were reactive in a modification of the direct test in which the incubation times were doubled. Sera obtained prior to transplantation from 3 patients who experienced either immediate or early rejection of their renal allografts contained antibodies detected by the antiglobulin test but not by direct microcytotoxicity tests against lymphocytes from a panel of unrelated, normal individuals. In addition, the antiglobulin test has been used to demonstrate that the humoral antibody response following transplantation of cardiac and renal allografts is heterogeneous with respect to formation of antibodies of the IgA, IgG and IgM classes.