Possible Use of Established Cell Lines for MLR Locus Typing

Authors

  • Erik A. J. Svedmyr,

    1. Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden
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  • Wolfgang Leibold,

    1. Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden
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    • 1

      Supported by the Deutsche Forschungsgemeinschaft. Present address: Institut fur Pathologie der Tierartzlichen Hochschule, Bischofsholer Damm 15, D3 Hannover, West Germany.

  • RICHARD A. GATTI

    1. Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden
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    • 2

      A recipient of a United States Public Health Service Research Career Development Award. Present address: Cedars of Lebanon Hospital, Dept. of Pediatrics, Los Angeles 90054, California, U.S.A.


Abstract

MLR histocompatibility typing was performed by in vitro stimulation of lymphocytes from various persons with fresh lymphocytes (irradiated) or with lymphoblastoid cell lines (mito-mycin-treated) derived from donors homozygous at the MLR locus. MLC responses to both types of stimulation correlated strongly suggesting that a) established lymphoblastoid line cells can express products of the MLR locus and b) fresh lymphocytes can be replaced by these long term lines in MLR typing. Two lines (LV-B and MS-B) derived from two MLR homozygous donors (LV and MS) were used throughout these experiments. It is possible that as additional lines are established from such MLR homozygous donors, a panel of typing cells could be created which would serve to standardize MLR typing and allow selection of unrelated but histocompatible donors for transplantation.

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