Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4)

Authors

  • X. Romero,

    1. Department of Cellular Biology and Pathology, Immunology Unit, Medical School, University of Barcelona and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
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  • D. Benítez,

    1. Servei d'Immunologia, Hospital Clínic and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
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  • S. March,

    1. Department of Cellular Biology and Pathology, Immunology Unit, Medical School, University of Barcelona and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
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  • R. Vilella,

    1. Servei d'Immunologia, Hospital Clínic and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
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  • M. Miralpeix,

    1. Almirall Prodesfarma S.A., Research Center, Barcelona, Spain
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  • P. Engel

    Corresponding author
    1. Department of Cellular Biology and Pathology, Immunology Unit, Medical School, University of Barcelona and Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.
      *Dr Pablo Engel
      Facultad de Medicina
      Departamento de Biología Celular y Anatomía Patológica
      Unidad de Inmunología
      C/Casanova 143
      08036 Barcelona, Spain
      Fax: +34 345 15272
      e-mail: engel@medicina.ub.es
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*Dr Pablo Engel
Facultad de Medicina
Departamento de Biología Celular y Anatomía Patológica
Unidad de Inmunología
C/Casanova 143
08036 Barcelona, Spain
Fax: +34 345 15272
e-mail: engel@medicina.ub.es

Abstract

Abstract The CD150 (SLAM) family consists of nine leukocyte cell-surface proteins involved in lymphocyte activation that belong to the immunoglobulin (Ig) superfamily. Six members of this family – CD84, CD150 (SLAM), CD229 (Ly9), CD244 (2B4), NTB-A, and CS1 – associate with adapter proteins – SLAM-associated protein (SAP) and EAT-2. SAP is a short intracellular molecule that is mutated in humans with X-linked lymphoproliferative disease. Flow cytometric analysis of the expression of CD84, CD150, CD229, and CD244 cell-surface receptors on several leukocyte and lymphocyte subsets was performed. CD84 and CD150 were present on thymocytes, mature T cells and antigen-presenting cells. The expression of CD84 and CD150 was high on memory T cells. CD150 expression was strongly up-regulated after cell activation. In contrast to CD84, CD150 was absent on resting monocytes and immature dendritic cells (DCs). CD229 presented a pattern of expression restricted to lymphocytes. CD244 was preferentially expressed on natural killer cells, CD8+ effector cells, resting monocytes, basophils, and eosinophils. We describe a broader distribution of CD84, CD150, CD229, and CD244 than previously reported and show that they are differentially expressed on hematopoietic cells. The heterogeneous expression of these receptors indicates that these molecules may play non-redundant functions in the regulation of both innate and adaptive immune responses.

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