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Strategies for evaluating B*18 allelic diversity by sequence-based typing applied to studies of a population from Singapore and African-Americans


*Carolyn Katovich Hurley, PhD
E404 Research Building
Georgetown University Medical Center
3970 Reservoir Road
NW Washington, DC 20007
Tel: 1 202 687 2157
Fax: 1 202 687 6440


Strategies to resolve B*18 alleles which carry a deletion in intron 1 close to the 5′ end of exon 2 relative to other HLA-B alleles or a null allele mutation in exon 1 and to resolve ambiguities among allele combinations including B*18 are described. B*18 allele frequencies from volunteer donors recruited for two hematopoietic stem cell registries show the presence of two alleles, B*180101 and B*1802, in a population from Singapore and only B*180101 in African-Americans.